ChIP-Seq and RNA-Seq reveal an AmrZ-mediated mechanism for cyclic di-GMP synthesis and biofilm development by Pseudomonas aeruginosa.
-
Expression of PA4843 (adcA) raises cellular c-di-GMP about 30-fold.
"expression of PA4843 in these strains generated nearly thirty fold more c-di-GMP"
-
Deleting adcA in an amrZ mutant removes its c-di-GMP accumulation and hyperbiofilm phenotype.
"Deletion of adcA in a ΔamrZ mutant eliminated the accumulation of c-di-GMP and the hyper biofilm phenotype"
The Pseudomonas aeruginosa diguanylate cyclase GcbA, a homolog of P. fluorescens GcbA, promotes initial attachment to surfaces, but not biofilm formation, via regulation of motility.
-
GcbA overexpression raises c-di-GMP to 228 pmol/mg versus under 35 for controls.
"PAO1/pJN- gcbA cultures on average contained 228 pmol of c-di-GMP per mg of total cell protein"
-
GcbA represses swimming and swarming motility.
"Inactivation of gcbA resulted in small, but significant increases in both swimming and swarming motilities, while overexpression of gcbA suppressed both forms of flagellum-driven motility"
The diguanylate cyclase GcbA facilitates Pseudomonas aeruginosa biofilm dispersion by activating BdlA.
-
GcbA DGC activity is needed for BdlA processing and for dispersal in response to many cues.
"plays an essential role in allowing biofilm cells to disperse in response to increasing concentrations of a variety of substances, including carbohydrates, heavy metals, and nitric oxide"
Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt