Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Combined Automated Annotation using Multiple IEA Methods
The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification.
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Immunopurification of intact human Complex I and mass-spectrometric identification resolved the human homologues of 42 Complex I polypeptides, including NDUFB8/ASHI, establishing NDUFB8 as a component of the complex.
"homologues of 42 polypeptides detected so far in the more extensively studied"
Knockdown of human COX17 affects assembly and supramolecular organization of cytochrome c oxidase.
Accessory subunits are integral for assembly and function of human mitochondrial complex I.
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Systematic gene-editing knockout of each of the 45 human Complex I subunits with quantitative proteomics showed that 25 accessory subunits are strictly required for assembly of a functional complex, and that loss of each subunit destabilizes other subunits in the same structural module. NDUFB8 is one of these accessory subunits.
"required for assembly of a functional complex and 1 subunit is essential for"
Architecture of Human Mitochondrial Respiratory Megacomplex I(2)III(2)IV(2).
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Cryo-EM of the human respiratory megacomplex assigned individual subunits of human Complex I, providing structural evidence for NDUFB8 as a Complex I subunit located in the inner membrane.
"reveals the precise assignment of individual subunits of"
Assembly of mammalian oxidative phosphorylation complexes I-V and supercomplexes.
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Review of OXPHOS assembly describing the human complexes as comprising core catalytic proteins plus a large number of supernumerary subunits with essential roles in assembly, regulation and stability.
"of 'supernumerary' subunits that play essential roles in assembly, regulation"
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
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High-confidence quantitative human mitochondrial proteome; source of the HTP mitochondrial-localization annotation for NDUFB8.
"Quantitative high-confidence human mitochondrial proteome and its dynamics"
cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed.
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Reports the cDNA sequences of the nuclear-encoded human Complex I subunits (completing characterization of the human complex), describing Complex I as an inner-membrane multiprotein complex that transports electrons from NADH to ubiquinone with coupled proton translocation.
"transport of electrons from NADH to ubiquinone, which is"
Complex I oxidises NADH to NAD+, reduces CoQ to CoQH2
Intermediate 1 binds HP subcomplex to form Intermediate 2
Peripheral arm subunits bind the 815kDa complex to form a 980kDa complex
Intermediate 2 binds MT-ND1:NDUFAF5:NDUFAF6 to form a 315kDa subcomplex
The MCIA complex, NDUFAF2-7 all dissociate from the 980kDa complex, resulting in Complex I
ND4, ND5 bind the 550kDa complex to form the 815kDa complex
The 315kDa subcomplex binds the 370kDa subcomplex to form the 550kDa complex
PITRM1 proteolyzes mitochondrial targeting peptides (presequences)