Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
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IBA annotations based on PANTHER phylogenetic analysis across bHLH transcription factor family
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Supports core annotations for transcription factor activity, E-box binding, nucleus localization, and neurogenesis processes
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
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Maps UniProt keywords (DNA-binding, Neurogenesis, Differentiation) to GO terms
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Produces generic annotations that often need refinement to more specific terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Gene Ontology annotation of human sequence-specific DNA binding transcription factors based on TFClass database
Electronic Gene Ontology annotations created by ARBA machine learning models
Impact of cytosine methylation on DNA binding specificities of human transcription factors
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Systematic HT-SELEX study profiling DNA binding specificities of 542 human transcription factors
"By analysis of 542 human TFs with methylation-sensitive SELEX (systematic evolution of ligands by exponential enrichment), we found that there are also many TFs that prefer CpG-methylated sequences"
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Provides experimental evidence for sequence-specific DNA binding activity
"systematic examination of the effect of an epigenetic DNA modification on human TF binding specificity"
A reference map of the human binary protein interactome
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Systematic Y2H screen identifying PFDN5 (prefoldin subunit 5) as NEUROG2 interactor
"three Y2H assay versions...that showed good sensitivity and low false positive rates when benchmarked against gold-standard positive and random reference sets"
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Represents high-throughput protein-protein interaction data
"The dataset, versioned HI-III-20 (Human Interactome obtained from screening Space III, published in 2020), contains 52,569 verified PPIs involving 8,275 proteins"
Deep research on NEUROG2 function in cortical neurogenesis
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NEUROG2 is the predominant neurogenin in cortical neurogenesis
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Binds E-box motifs (CANNTG) with preference for CAT-containing sequences
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Forms heterodimers with E-proteins for DNA binding
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Direct targets include Dll1, Dll3, Rnd2, Neurod1, Neurod2, Lhx2
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Functions in nucleus with pioneer-like chromatin activity
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Phosphorylation at T149 modulates chromatin remodeling versus transactivation
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LHX2 suppresses NEUROG2 in progenitors to regulate dendritic morphogenesis
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Human-specific targets include PPP1R17 and ECM genes
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Upregulated in MYCN-driven neuroendocrine prostate cancer
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Enriched in cortical intermediate progenitors, scarce in postmitotic neurons