Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Cloning and nucleotide sequence of the Pseudomonas aeruginosa nfxB gene, conferring resistance to new quinolones.
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Cloning of nfxB; a point mutation in the predicted HTH region affects quinolone permeability.
"The gene nfxB is one of the genes which affect the cell membrane permeability of quinolones in Pseudomonas aeruginosa PAO"
Mutation of nfxB causes global changes in the physiology and metabolism of Pseudomonas aeruginosa.
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Most nfxB mutant phenotypes are indirect, caused by MexCD-OprJ overexpression.
"suggesting that inappropriate overexpression of a functional MexCD-OprJ efflux pump causes pleiotropic changes"
Functional characterization of the NfxB repressor of the mexCD-oprJ multidrug efflux operon of Pseudomonas aeruginosa.
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NfxB binds an inverted-repeat region upstream of mexCD-oprJ and acts as a tetramer.
"NfxB binds upstream of the mexCD-oprJ transcription initiation site to a region containing two inverted repeats, both of which are required for binding"
Purification and characterization of the Pseudomonas aeruginosa NfxB protein, the negative regulator of the nfxB gene.
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Purified NfxB binds upstream of nfxB (gel retardation, DNase I footprinting).
"NfxB specifically binds upstream of the nfxB coding region as demonstrated by gel retardation and DNase I footprinting"
Overexpression of the mexC-mexD-oprJ efflux operon in nfxB-type multidrug-resistant strains of Pseudomonas aeruginosa.
nfxB (PA4600) review notes