Characterization of the SOS regulon of Caulobacter crescentus.
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The imuA promoter is a LexA-dependent SOS promoter in C. crescentus, confirmed by site-directed mutagenesis of its operator.
"which was confirmed by site-directed mutagenesis studies of the imuA promoter"
An SOS-regulated operon involved in damage-inducible mutagenesis in Caulobacter crescentus.
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imuA, imuB and dnaE2 are co-expressed from a LexA-regulated operon and all three are required for UV- and mitomycin C-induced mutagenesis.
"The results show that the dnaE2 operon is upregulated in response to DNA damage in a recA-dependent manner, and demonstrate that all three genes that compose this expression unit are involved in UV and mitomycin C-induced mutagenesis."
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An imuA deletion is extremely sensitive to mitomycin C.
"The single mutants imuA, imuB and dnaE2 mutants are extremely sensitive to this condition, revealing that this operon plays a major role in the tolerance to the mit C-induced DNA lesions."
UniProtKB entry for imuA (Q9A3J1)
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UniProt records ImuA as required, with DnaE2 and ImuB, for error-prone processing of DNA lesions, and assigns the ImuA family IPR017026.
"DR InterPro; IPR017026; ImuA."
Myxococcus xanthus translesion DNA synthesis protein ImuA is an ATPase enhanced by DNA.
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Purified Myxococcus ImuA has DNA-stimulated ATPase activity and directly binds ImuB; these are comparator results, not measurements on Caulobacter.
"Here we carry out biochemical characterization of ImuA and a truncation of ImuB from Myxococcus xanthus."
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The Myxococcus result supplies a specific experimental hypothesis for Caulobacter without proving activity conservation.
"We find that ImuA is an ATPase, with ATPase activity enhanced in the presence of DNA."