DCAF11 PN Consistency Notes
- Generated: 2026-06-18
- Project: PROTEOSTASIS
- Scope: PN consistency rereview against local AIGR review and available deep-research artifacts
- UniProt: Q8TEB1
- AIGR review status: COMPLETE
- Review batch: proteostasis-batch-2026-06-07
- Batch change status: added
Source Files Checked
Deep Research Files
- No
*-deep-research*.md file found in this gene directory.
AIGR Review Snapshot
- Description: DCAF11 (DDB1- and CUL4-associated factor 11; also called WD repeat-containing protein 23, WDR23) is a WD40-repeat protein that serves as a substrate-recognition receptor of the CRL4 (DDB1-CUL4-RBX1) cullin-RING E3 ubiquitin ligase. Through a conserved WDXR motif it docks onto the DDB1 adaptor of CUL4A- and CUL4B-based complexes and uses its C-terminal WD40 beta-propeller to present specific substrates for polyubiquitination and proteasomal degradation. Characterized substrates include the NRF2/NFE2L2 transcription factor (via the DIDLID sequence of its Neh2 domain), which links DCAF11 to the cellular antioxidant/oxidative-stress response, and the centromeric histone variant CENP-A, whose phospho-Ser68-primed degradation DCAF11 mediates to maintain centromere identity. The protein localizes predominantly to the nucleoplasm, consistent with its nuclear substrates. The orthologous receptor in C. elegans (WDR-23) controls the NRF/Nrf2 ortholog SKN-1, and DCAF11 has also been exploited as a recruited ligase in covalent molecular-glue targeted protein degradation.
- Existing/core annotation action counts: ACCEPT: 10; KEEP_AS_NON_CORE: 3; MARK_AS_OVER_ANNOTATED: 8; MODIFY: 1
PN Consistency Summary
- Consistency: Fully consistent. Deep-research notes, review, and PN all describe DCAF11 as a CRL4 substrate receptor (WD40 + WDxR docking on DDB1) with two validated substrates: NRF2/NFE2L2 (PMID:31586112) and CENP-A (PMID:34758320). Better characterized than DCAF10. No contradictions.
- PN story / NEW pressure: Already captured. Unlike DCAF10, the DCAF11 review already MODIFIES a bare protein-binding annotation to GO:1990756 (proposed_replacement_terms on the PMID:16949367 DDB1/CUL4 IPI) AND uses GO:1990756 as the molecular_function of its first core_function. PN's projected GO:1990756 thus duplicates an MF the review already asserts. No additional ADD needed. (PN flags it new_to_goa because GOA proper still only has bare protein binding — the review's MODIFY is the mechanism to introduce it.) Substrate-adaptor MF only; no catalytic RING (distinct from COP1).
- Evidence alignment: PN cites 17588513 (DCAF review). Review's gene-specific evidence (PMID:16949367 founding DCAF, 16964240 DDB1-CUL4A architecture, 31586112 NRF2, 34758320 CENP-A) does not directly overlap the single PN citation but is concordant at the family/mechanism level.
- Verdict: Consistent; mapping sound; no edits. GO:1990756 already in review (core_function + MODIFY); PN projection is redundant-but-correct.
Full Consistency Review
- UniProt: Q8TEB1 (WDR23) · batch: proteostasis-batch-2026-06-07 · review status: COMPLETE
- PN placement:
UPS|E3 ubiquitin and UBL ligases|Cul4A/Cul4B substrate receptor|WD40|other ; PN-node mapping: group node Cul4A/Cul4B substrate receptor mapped → GO:1990756 (ok_for_propagation, new_to_goa); class context_only (GO:0061630, too_broad).
- Consistency: Fully consistent. Deep-research notes, review, and PN all describe DCAF11 as a CRL4 substrate receptor (WD40 + WDxR docking on DDB1) with two validated substrates: NRF2/NFE2L2 (PMID:31586112) and CENP-A (PMID:34758320). Better characterized than DCAF10. No contradictions.
- PN story / NEW pressure: Already captured. Unlike DCAF10, the DCAF11 review already MODIFIES a bare protein-binding annotation to GO:1990756 (proposed_replacement_terms on the PMID:16949367 DDB1/CUL4 IPI) AND uses GO:1990756 as the molecular_function of its first core_function. PN's projected GO:1990756 thus duplicates an MF the review already asserts. No additional ADD needed. (PN flags it new_to_goa because GOA proper still only has bare protein binding — the review's MODIFY is the mechanism to introduce it.) Substrate-adaptor MF only; no catalytic RING (distinct from COP1).
- Mapping strategy: Correct. Group → GO:1990756 substrate-adaptor MF is right; class GO:0061630 correctly too_broad. Projection is precisely scoped, not over-reaching (contrast the rejected TOMM20/HSPA8/RAB7A broader-term cases). No mapping change.
- Evidence alignment: PN cites 17588513 (DCAF review). Review's gene-specific evidence (PMID:16949367 founding DCAF, 16964240 DDB1-CUL4A architecture, 31586112 NRF2, 34758320 CENP-A) does not directly overlap the single PN citation but is concordant at the family/mechanism level.
- Verdict: Consistent; mapping sound; no edits. GO:1990756 already in review (core_function + MODIFY); PN projection is redundant-but-correct.
PN Dossier Context
- review_batch: proteostasis-batch-2026-06-07
- review_yaml: genes/human/DCAF11/DCAF11-ai-review.yaml
- PN workbook rows: 1
PN row 1: Ubiquitin Proteasome System | E3 ubiquitin and UBL ligases | Cul4A/Cul4B substrate receptor | WD40 | other
- UniProt: Q8TEB1
- In branches: UPS
- Signature domains: (none)
- Auxiliary domains: IPR001680
- PN references (titles):
- PN-node mapping records (path + ancestors):
- [subtype] Ubiquitin Proteasome System|E3 ubiquitin and UBL ligases|Cul4A/Cul4B substrate receptor|WD40|other
status=no_mapping scope= GO=[]
rationale: Reviewed as a narrower substrate-receptor, adaptor, domain, or family subdivision already covered by the curated parent adaptor/receptor mapping. No additional direct GO mapping is needed at this node.
- [type] Ubiquitin Proteasome System|E3 ubiquitin and UBL ligases|Cul4A/Cul4B substrate receptor|WD40
status=no_mapping scope= GO=[]
rationale: Reviewed as a narrower substrate-receptor, adaptor, domain, or family subdivision already covered by the curated parent adaptor/receptor mapping. No additional direct GO mapping is needed at this node.
- [group] Ubiquitin Proteasome System|E3 ubiquitin and UBL ligases|Cul4A/Cul4B substrate receptor
status=mapped scope=ok_for_propagation_to_go GO=[GO:1990756 ubiquitin-like ligase-substrate adaptor activity]
rationale: This PN group captures substrate receptors/adaptors for cullin/UBL ligase systems. The shared GO molecular-function target is ubiquitin-like ligase-substrate adaptor activity.
- [class] Ubiquitin Proteasome System|E3 ubiquitin and UBL ligases
status=context_only scope=too_broad_to_propagate GO=[GO:0061630 ubiquitin protein ligase activity]
rationale: This class is a genuine E3-ligase context, but its descendants include catalytic ligases, cullin scaffolds, substrate receptors, adaptors, cofactors, regulators, and UBL modifier systems. A class-level propagation would over-annotate.
- [branch] Ubiquitin Proteasome System
status=no_mapping scope= GO=[]
rationale: Reviewed as the top-level UPS branch. It is a project taxonomy umbrella rather than a direct GO assertion; UPS propagation must come from manually curated child nodes.
Projected GO annotations (1)
- GO:1990756 ubiquitin-like ligase-substrate adaptor activity | scope=ok_for_propagation_to_go | goa_status=new_to_goa | from=Ubiquitin Proteasome System|E3 ubiquitin and UBL ligases|Cul4A/Cul4B substrate receptor
Note
This file is generated from the current PROTEOSTASIS phase-1 dossier and local gene-review artifacts. Edit the source review, PN mapping, or dossier rather than this generated note when correcting the underlying curation.