bio2 (biotin synthase, UniProt O59778, SPCC1235.02) - notes

Identity

• PomBase bio2 = SPCC1235.02 = UniProt O59778; ortholog of S. cerevisiae BIO2 (P32451) and E. coli BioB.

Function

• Radical-SAM biotin synthase EC 2.8.1.6, conversion of dethiobiotin to biotin, final step of biotin synthesis [UniProt:O59778 "FUNCTION: Catalyzes the last step of biotin biosynthesis, the"].
• Experimental: S. pombe bio2 complements a S. cerevisiae bio2 mutant PMID:10525840. This underlies PomBase IGI rows (with SGD BIO2).
• Cofactors by similarity: [4Fe-4S] (radical SAM) and [2Fe-2S] (sulfur donor) clusters [UniProt:O59778].

Localization

• Mitochondrion HDA PMID:16823372; consistent with S. cerevisiae BIO2 mitochondrial localisation.

GO-CAM

• gomodel:67c10cc400002026: SPCC1235.02 enables GO:0004076 part_of GO:0009102 (IGI PMID:10525840 + IBA); no occurs_in given.