DYSF source and evidence guide

This guide identifies the evidence actually examined and separates ordinary publication-cache contents from independently recovered originals. It is a locator, not an additional biological experiment. All 45 imported assertions remain intact; 15 products, two core activities and zero NEW annotations are represented. All support is reference-only, with no newly authored publication excerpts.

Biochemical and product evidence

Source Actual evidence and access Limits
PMID:11959863(https://doi.org/10.1074/jbc.M201858200) Authentic complete abstract positively reports calcium-sensitive phospholipid binding and a mutant effect. Publisher/JBC attempts did not recover full original text. Detailed unseen construct/figure claims are not asserted.
PMID:24239457(https://pmc.ncbi.nlm.nih.gov/articles/PMC5993433/) Complete operative Methods/Results: bacterial expression of human canonical C2A 1–125 and alternative C2Av1 1–126 fragments; calcium/lipid assays; full-length surface experiments. C2Av1 Cys2Ala and tag/protease handling are construct details. Surface experiments shown are in mouse C2C12; the corresponding HEK293 result is data not shown. Alternative exon 1 is a sequence class, not a unique complete natural isoform.
PMID:24461013(https://pmc.ncbi.nlm.nih.gov/articles/PMC3907215/) Protein-expression, ITC and liposome Methods plus corresponding Results were read. Human AF075575.1-derived isolated C2 domains show calcium binding and acidic-lipid binding, including EDTA baseline and calcium enhancement. No intact-product repair assay or equivalence across all 15 products follows from these isolated domains.
UniProt O75923 All 15 official FASTAs were independently reconstructed from the displayed sequence and stated variation features. VSP_035924 occurs in products 8–14; only product 15 lacks the canonical membrane anchor. Historical naming in 16896923 and 19221801 was checked at abstract scope.

Interactions and partner classes

PMID:23414517(https://pmc.ncbi.nlm.nih.gov/articles/PMC3610214/) was read at full operative Methods/Results scope, with the genuine supplementary archive. The public source audit reproduces the exact six target-pair rows and source-specific validation fields. File 2044-5040-3-3-S1.xls is internally named Table S2; rows 399, 402, 403, 405, 469 and 1137 identify MYBPC1, MYOM1, NEB, TTN, MYOM2 and SGCG, respectively. The independent bait file S2.xls, internally Table S1, gives DYSF L2–I485 at row 12. These mismatched source labels are preserved.

The IntAct query contains 101 records. Publication-and-partner joins select 34 records; all 68 participant reference checksums match saved sequences. The complete record identifiers, taxa, roles and feature strings are in the replay output. These checks authenticate reference participants, not complete experimental inserts or unique natural isoforms. Human Y2H libraries, mouse co-IP and human biopsy proximity imaging remain separate. Negative colocalization does not itself negate a positive interaction assay.

Independent official partner-class links and the exact current definitions of GO:0008092 and GO:0042826 are included in RESULTS.md and the saved inputs. Class refinements do not assign the partner's activity to dysferlin. SGCG's membrane/DGC role remains distinguished from an established actin-, microtubule- or intermediate-filament structural component.

PMID:24687993(https://pmc.ncbi.nlm.nih.gov/articles/PMC4062822/) was independently recovered and read through human-cell differentiation, endogenous co-IP and operative assay Methods. Positive dysferlin/HDAC6 and dysferlin/FAM65B-RIPOR2 associations are separate from the deacetylase assay. The effective normal cache remains abstract-only. PMID:17185750(https://doi.org/10.1096/fj.06-6628com) remains abstract-only despite bounded original attempts; its positive reported AHNAK interaction and official AHNAK/AHNAK2 records support preservation with detailed assay limits. Empty IntAct joins for these sources do not establish absence.

Location and contextual evidence

Source Actual scope Interpretation boundary
10196375, 10496277 Complete authentic abstracts support surface-membrane association. No full-paper or isoform-specific detail was inferred.
20595382 Full operative Methods/Results in C2C12 support surface localization and an early/late endocytic itinerary for full-length and mutant tagged constructs. Host is mouse. Gift-construct provenance is not complete natural-human-product authentication; accelerated mutant trafficking is distinct from exclusivity.
26707827 Authentic abstract and independently indexed publisher Results support PM/Rab7-endosome observations in the specified transfected-cell contexts. Complete Methods and figure pixels were unavailable. Rab7 late endosomes are mostly separate from LAMP1 structures; no wholesale lysosomal relabeling is made.
17363620, 17554076 Complete abstracts provide myogenic trafficking and human placental context. Used as corroboration, with no unseen full-text claim.
HPA subcellular page, XML HPA017071 target metadata were inspected, including Rh30 plasma-membrane staining. Plasma membrane is supported; centriolar satellite and midpiece are uncertain. Overall approved reliability does not upgrade uncertain locations. No image pixels were independently scored; U2OS lacks staining in the saved record.
Reactome R-HSA-5263633 The exact input R-HSA-5263626 resolves to human O75923 residues 1–2080 in cytoplasmic vesicle membrane. This is a curated assembly/pathway state, not a new measured transport rate or purified interaction. The 11532985 abstract provides independent muscle association context.
18276788 Independently recovered complete operative Methods/Results distinguish human patient monocytes, TNF treatment, tissue observations and mouse J774 knockdown. Untreated human phagocytosis was not significantly increased. No direct receptor/Rho mechanism is assigned from the proposed model.
19056867 Original Table 1 and Methods identify DYSF with one unique peptide/one spectrum in pooled human urine; linked NHLBI data identify NP_003485. Gene-level detection does not discriminate all products or establish active extracellular function. The effective cache is abstract-only; the separately read original supplies the target table.
23533145 Full main Methods/Results describe human EPS-urine exosome enrichment and proteomics. The valid DYSF supplementary row was not recovered: small XML/HTML/error responses are not spreadsheets. The positive curated assertion is retained with independent urinary-exosome corroboration.

Propagation sources

The replay verifies the 194-entry PTHR45911 tree, alignment, exact 2,080-residue target sequence and whole IBD source. The current geneinfo lookup and UniProt family pointer disagree; this is resolved by target identity, not by changing an imported PTN. Both imported nodes are on the target path. The normal PTHR12546 lookup output is retained as private acquisition provenance and is not substituted for the verified target tree.

PTN002473508 grounds calcium binding, with legitimate target experimental evidence among its descendants. PTN000413636 grounds the two unresolved synaptic assertions. Rat RGD:1305199 resolves to Mctp1: its 26195140 abstract supports neuronal vesicle/presynaptic observations, but the full original was not obtained. Fly FBgn0034389 resolves to Mctp; the 28485711 original was read for presynaptic ER localization, release/homeostatic phenotypes and neuronal rescue. These are donor experiments, not human DYSF experiments. MCTP/ferlin differences raise specialization uncertainty without proving loss or invalid ancestry. The mouse dysferlin donor Q9ESD7 is an ortholog with experimental location annotation, independently corroborated by target trafficking sources. Its 12736685 source was checked at complete abstract scope: mouse muscle shows dysferlin-associated membrane patches and vesicle accumulation; full operative original text was not independently read.

Electronic contexts were checked against the actual target FunFam/InterPro features, lineage and relevant ARBA condition groups: ARBA00089298, ARBA00089297, ARBA00089681, ARBA00087396 and ARBA00033481. Only matching branches are claimed. InterPro IPR006614/IPR012560/IPR012561 legitimately map a broad membrane term; refinement uses independently supported PM and internal-vesicle pools. GO_REF entries describe methodology and are individually assessed as LOW biological relevance, rather than treated as target experiments.

Effective publication-cache inventory

The table below is generated from the actual selected cache headers. A true flag means text is present in that cache, not that every paragraph or supplement was examined. A false flag does not erase a separately recovered original described above. Existing repository publication bytes take precedence over fresh variants and are preserved exactly.

PMID Effective full-text flag SHA-256
10196375 false 84229925ae2356a720a7aa71c58f21cd4f173c5b7fc422a26f4debb8783f79f5
10496277 false 6a9a9b97761b0ca488be0599947d510733ea168f3f7e172420b4e456ee41096a
11532985 false 727f882b261652b26f47c82422c8a0846e0318709bc209de6342139ee3bd60a1
11959863 false f06f57d129aacbfda8edd2536e0b3f203573496d6681fc838c850febd57a6503
12736685 false cb557e6a5792e0109ba453095626b5b8053e45e9073a672b399f276d640b65eb
16896923 false 7fefe481c2e0155de7f35b8cf0687253fe653dfcb6a2710befe2da6335a07192
17185750 false 9895f71f8271c0d17ec74ffebe367c222156bc3afa5937eac5803260e650f8b9
17363620 false 6adc90f9aefd7ea8495fd01338050fc94fd42113acf8002f2fd2966b4f5ae2c3
17554076 false 2233abb1b93c41b69344d32f318ce48620edef4f28992c6bae9c37374a1e84b5
18276788 false 1ed4cdaf974df5fb9d6d64faa234591b2833d7451805b40625bddca27fa0a8ca
19056867 false 90ceb930bcd3ba708af5c0630dfcc028133affae4b73bd0409c758d54b43f2c4
19221801 false 4d7cad37f142b709e9c947dea0d55e632be5c6f633fb6a53e11ee8b5045d2ee4
20595382 true 7149f7809db9d8f79aefac39968c96a2974c05221e39aa1f6e43c6b100cf486c
23414517 true 0d08e0c6a78953ef41a9c8dc98c07dc7a7b14bc963fa55b306bed11b983d1cae
23533145 true f347293c23617bd56d83e1ae9bc3ad8a65e540fdbf4aff13bd0e918ec598a17e
24239457 true 24aa88323c0058c04dc24a1847846c52ea99a9fb9c1ae9a8d66551d3c31b7b96
24461013 true e05971faedb09f1718b311decf303f3bde21f99ca75d872638cfa8de797dfa6c
24687993 false 90b864283b905164800a21c5b120c16926acd7e042bec9fa584f7f4561320df9
26195140 false 2968817035e345b196f115dffd7d82131fb6a93ca6e4f5916173c9aa75f20de3
26707827 false c27ddff9927b85c2aeaafc01da0fd2ad0acc6af7af39aa3f16355b69bfe93572
28485711 true ace6fb4adfdd002a068aeee7e8fd1f1def73a3942cdf730f265b9855bd1ad8e9

The source audit includes exact input URLs, hashes, a locked dependency and executed saved outputs. Reference-only links from the review distinguish primary support, computed source joins and this locator guide. The normal research-provider failures are documented in the notes; they are not represented as completed research reports.

Follow-up source access

PMID:24967968(https://pubmed.ncbi.nlm.nih.gov/24967968/): normal full-text cache; Results on patient myoblasts and Methods on cell origin/laser injury were read. The human comparison uses one patient and an unmatched control; mechanistic lysosome experiments use mouse cells.

PMID:27226605(https://pmc.ncbi.nlm.nih.gov/articles/PMC4938179/): normal cache is abstract-only; the original PMC HTML Results and Methods were read independently. The experiment measures lipid mixing, including an inner-leaflet control, rather than directly measuring wound closure. The soluble human construct is reported as residues 1–1965; no current natural-product identity is assigned.

The repair audit preserves the current ontology and comparator query results. No newly authored quotation or machine-cache flag change was introduced.