Use of the ND evidence code for Gene Ontology (GO) terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Global analysis of protein localization in budding yeast.
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Genome-wide GFP-fusion localization assigned OCA6 to the cytoplasm; this is the basis of the HDA cytoplasm annotation.
"we describe the construction and analysis of a collection of yeast strains expressing full-length, chromosomally tagged green fluorescent protein fusion proteins."
Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus.
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OCA6 was identified in a genome-wide yeast single-gene-deletion screen as one of ~100 host genes whose loss altered Brome mosaic virus (BMV) RNA replication; this is the basis of the UniProt "Required for replication of BMV" function statement.
"This functional genomics approach revealed nearly 100 genes whose absence inhibited or stimulated BMV RNA replication and/or gene expression by 3- to >25-fold."
Phylogenetic and genetic linkage between novel atypical dual-specificity phosphatases from non-metazoan organisms.
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OCA6 is one of the fungal PFA-DSP-related atypical dual-specificity phosphatase proteins of S. cerevisiae; the closest characterized homolog is the active PFA-DSP Siw14/Oca3.
"Bioinformatic analysis revealed the existence of novel PFA-DSP-related proteins in fungi (Oca1, Oca2, Oca4 and Oca6 in Saccharomyces cerevisiae) and protozoa"
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OCA6, together with the other OCA-family members, is involved in the yeast response to caffeine and rapamycin stresses, and oca6 caffeine sensitivity is suppressed by overexpression of the active family member Siw14/Oca3 (genetic linkage).
"Oca1, Oca2, Siw14/Oca3, Oca4, and Oca6 were involved in the yeast response to caffeine and rapamycin stresses."
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Within the family, catalytic activity is not uniform: Siw14/Oca3 is an active phosphatase in vitro while no activity could be detected for Oca1 — direct catalytic activity of OCA6 itself was not demonstrated.
"Siw14/Oca3 was an active phosphatase in vitro, whereas no phosphatase activity could be detected for Oca1."
A multidimensional chromatography technology for in-depth phosphoproteome analysis.
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OCA6 is phosphorylated on Thr2, detected by large-scale mass-spectrometry phosphoproteomics.
"A multidimensional chromatography technology for in-depth phosphoproteome analysis"
Identification of a Saccharomyces cerevisiae gene that is required for G1 arrest in response to the lipid oxidation product linoleic acid hydroperoxide.