OpenScientist hypothesis run: Rv0311 intein / peptidase catalytic-block check
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Refutes the predicted intein-mediated protein splicing (GO:0016539) and autoproteolytic peptidase (GO:0008233) functions; Rv0311 lacks all intein catalytic residues, has no intein domain or fold, and is a standalone gene, so the PROSITE PS00881 match is a historical false positive.
"no structural similarity to any known intein fold (Foldseek: zero intein matches among 660 structural hits)"
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry
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Rv0311 protein is detected in multiple cellular fractions including membrane and culture filtrates
"we identified 3176 proteins from Mycobacterium tuberculosis representing ∼80% of its total predicted gene count"
Deep Research Report for Rv0311 gene function analysis
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Rv0311 contains a protein splicing signature motif suggesting intein-like activity
"Rv0311's sequence contains a conserved protein splicing (intein) signature (PROSITE pattern PS00881) ... includes key residues typically required for protein splicing"
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Rv0311 is essential for growth in minimal media but not in rich media
"Sassetti et al. (2003) found Rv0311 as essential for growth in vitro (on minimal medium 7H10 agar) ... DeJesus et al. (2017) with enriched media reported Rv0311 as non-essential under rich laboratory conditions"
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Rv0311 is required for virulence particularly in CNS infections
"Be et al. (2008) found an insertion mutant in the Rv0311 ortholog that was significantly attenuated in the brain of mice (a model of TB meningitis)"
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Rv0311 is regulated by stress response factors SigH and WhiB5
"ChIP-Seq analyses have identified transcription factors that bind near the Rv0311 promoter ... SigH (Rv3223c) ... WhiB5 (Rv0022c)"