Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
UniProtKB Q9S7E9 (GGAT2) entry
Proteomic analysis of leaf peroxisomal proteins in greening cotyledons of Arabidopsis thaliana.
Alanine aminotransferase homologs catalyze the glutamate:glyoxylate aminotransferase reaction in peroxisomes of Arabidopsis.
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Purified recombinant GGT2 catalyses Glu:glyoxylate, Glu:pyruvate and Ala:2-oxoglutarate transaminations with measured kinetics, and AGT activity; no SGT or AspAT activity.
"Recombinant GGT2 was soluble when expressed in E. coli ; lysates of bacteria expressing this construct exhibited significantly increased levels of GGT, GPT, and AKT activity compared with empty vector controls"
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GGT2 is far less represented in EST collections than GGT1.
"Only two potential EST sequences corresponding to GGT2 were discovered, including a single potential full-length cDNA."
Identification of photorespiratory glutamate:glyoxylate aminotransferase (GGAT) gene in Arabidopsis.
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AOAT2 (GGAT2) carries a PTS1 but is a minor isoform in green leaves; the GFP and knockout work concern AOAT1.
"The expression analysis of mRNA encoding AOATs and EST information suggested that AOAT1 was the major protein in green leaves."
Glutamate:glyoxylate aminotransferase modulates amino acid content during photorespiration.
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GGAT2 promoter-GUS is strongly expressed in leaves; GGAT2 mRNA rises after light exposure while GGAT1 mRNA falls, with constant leaf GGAT activity.
"After light exposure, the levels of GGAT1 mRNA decreased and those of GGAT2 mRNA increased rapidly"
Alanine aminotransferase catalyses the breakdown of alanine after hypoxia in Arabidopsis thaliana.
Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms.
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The second GGT isoform (GGAT2) is identified by isoform-specific peptides in mature leaf peroxisomes, less abundant than GGT1.
"Of the two isoforms each of Glu-glyoxylate aminotransferase (GGT) and peroxisomal malate dehydrogenase (pMDH), GGT1 (At1g23310; SKM>) and pMDH2 (At5g09660; RIx 5 HL) dominated over the second isoform, both of which were identified as well"
Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.
Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis.
Analysis of the Arabidopsis cytosolic proteome highlights subcellular partitioning of central plant metabolism.
Photorespiration.
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Both GGAT loci encode peroxisomal GGATs; GGAT2 is induced at the start of the photoperiod and both are thought to contribute to leaf GGAT content; residual GGAT2 activity may not fully explain the weak ggat1 phenotype.
"Whereas Ggat 2 is induced at the beginning of the photoperiod, Ggat 1 is rather repressed."
A proteomic strategy for global analysis of plant protein complexes.
Arabidopsis thaliana ggt1 photorespiratory mutants maintain leaf carbon/nitrogen balance by reducing RuBisCO content and plant growth.
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ggat1 mutants in air accumulate glyoxylate despite the presence of GGAT2, so GGAT2 cannot compensate for GGAT1 loss.
"When compared to wild-type (WT) plants, air-grown ggt1 plants exhibited glyoxylate accumulation"
Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.
Discovering the RNA-Binding Proteome of Plant Leaves with an Improved RNA Interactome Capture Method.