Cloning of Octopus cephalotocin receptor, a member of the oxytocin/vasopressin superfamily.
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CTR1 cloned from octopus brain; encodes 397 amino acid GPCR
"We cloned the cDNA of an orphan receptor from Octopus brain and found it to encode a polypeptide of 397 amino acids that displays sequences characteristic of G-protein coupled receptors."
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Specifically activated by cephalotocin via inositol phosphate/Ca2+ pathway
"Xenopus oocytes that express the orphan receptor responded to the application of CT by an induction of membrane Cl(-) currents coupled to the inositol phosphate/Ca(2+) pathway."
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Not activated by octopressin or vertebrate OT/VP peptides
"OP and the other members of the oxytocin/vasopressin superfamily did not activate this receptor."
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Expression in CNS, peripheral nervous system, pancreas, oviduct, ovary
"Expression of CTR mRNA in Octopus was detected in the central and the peripheral nervous systems, the pancreas, the oviduct and the ovary."
Novel evolutionary lineages of the invertebrate oxytocin/vasopressin superfamily peptides and their receptors in the common octopus (Octopus vulgaris).
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Reports CTR2 and OPR as additional OT/VP receptors in octopus
"In the present study, we have identified an additional CTR, CTR2, and a novel OP receptor, OPR."
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Confirms CTR1 specificity for cephalotocin
"Previously, we cloned a GPCR (G-protein-coupled receptor) specific to CT [CTR1 (CT receptor 1)]."
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Ligand-receptor selectivity depends on peptide residues at positions 2-5
"The amino acid residues of OP and CT at positions 2-5 were presumed to play crucial roles in the binding selectivity to their receptors."
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Invertebrate OT/VP receptors not activated by vertebrate OT/VP peptides
"These invertebrate receptors are not activated by vertebrate OT/VP family peptides."
An Octopus-Derived Peptide with Antidiuretic Activity in Rats.
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Cephalotocin acts as selective agonist of human AVP V1b and V2 receptors
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Cephalotocin has antidiuretic activity in rats
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Demonstrates cross-species pharmacology of OT/VP superfamily peptides
Comparative and Evolutionary Physiology of Vasopressin/Oxytocin-Type Neuropeptide Signaling in Invertebrates.
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Three major functional themes recur for OT/VP-type neuropeptides in invertebrates: reproduction, feeding/metabolism, and water/salt homeostasis
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The physiological function of cephalotocin in octopus is still not fully elucidated despite known receptor distribution
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The existence of two cephalotocin receptors (CTR1 and CTR2) invites questions about whether they have redundant or distinct roles
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Cephalotocin does not elicit acute effects on muscle tone or osmotic balance, suggesting its primary roles are in reproduction, metabolism, and neuromodulation
Gene Ontology annotation through association of InterPro records with GO terms
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods