Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA.
Activation state-dependent interaction between Galphai and p67phox.
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Gbetagamma-p67phox association is indirect via Galphai.
"Based on these data, we concluded that the association between Gβγ and p67 phox is indirect and mediated through the inactive, GDP-bound Gαi."
Huntingtin interacting proteins are genetic modifiers of neurodegeneration.
Integral and associated lysosomal membrane proteins.
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
The cationic region of Rhes mediates its interactions with specific Gbeta subunits.
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
KIF14 negatively regulates Rap1a-Radil signaling during breast cancer progression.
In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
X-ray structure of the mammalian GIRK2-βγ G-protein complex.
Proteomic analysis of cerebrospinal fluid extracellular vesicles: a comprehensive dataset.
A quantitative chaperone interaction network reveals the architecture of cellular protein homeostasis pathways.
A proteome-scale map of the human interactome network.
Comprehensive analysis of heterotrimeric G-protein complex diversity and their interactions with GPCRs in solution.
Phase-plate cryo-EM structure of a class B GPCR-G-protein complex.
Architecture of the human interactome defines protein communities and disease networks.
Cryo-EM structure of the serotonin 5-HT(1B) receptor coupled to heterotrimeric G(o).
Beta-subunits of the human liver Gs/Gi signal-transducing proteins and those of bovine retinal rod cell transducin are identical.
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Human Gs/Gi beta is identical to transducin beta.
"This work proves for the first time that the beta-subunits of all signal-transducing G-proteins, including transducin, are the same."
A reference map of the human binary protein interactome.
Cryo-electron microscopy structure of the glucagon receptor with a dual-agonist peptide.
Structure of a D2 dopamine receptor-G-protein complex in a lipid membrane.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Structural insights into the lipid and ligand regulation of serotonin receptors.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Structural basis for recognition of anti-migraine drug lasmiditan by the serotonin receptor 5-HT(1F)-G protein complex.
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Activation and allosteric regulation of the orphan GPR88-Gi1 signaling complex.
Multimodal cell maps as a foundation for structural and functional genomics.
Mitogen-activated protein kinase activation requires two signal inputs from the human anaphylatoxin C5a receptor.
Phosphorylation-dependent activation of the Ras-GRF/CDC25Mm exchange factor by muscarinic receptors and G-protein beta gamma subunits.
Binding of Gbeta/gamma to GIRK/Kir3 channels
Association of GABA B receptor with G protein beta-gamma subunits
Activation of GIRK/Kir3 Channels
The receptor:G-protein complex dissociates
Thrombin-activated PARs activate G12/13
Thrombin-activated PARs activate Gq
The high affinity receptor complex binds to G-protein
The receptor:G-protein complex releases GDP
The receptor:G-protein complex binds GTP
G-protein beta-gamma subunits rebind the alpha-GDP subunit
Gt-GTP dissociates to GNAT1-GTP and GNB1:GNGT1
Liganded Gs-activating GPCR acts as a GEF for Gs
Liganded Gq/11-activating GPCRs act as GEFs for Gq/11
Liganded Gi-activating GPCR acts as a GEF for Gi
Activated P2Y purinoceptor 12 binds G-protein Gi
Gi activation by P2Y purinoceptor 12
Dissociation of the P2Y purinoceptor 12:Gi complex
Gbeta:gamma recruits PI3K gamma
G beta:gamma activated PI3Kgamma converts PIP2 to PIP3
Activated prostacyclin receptor binds G-protein Gs
Gs activation by prostacyclin receptor
WNT:FZD complex promotes G-protein nucleotide exchange
G-protein subunits dissociate from WNT:FZD complex
Thrombin-activated PARs binds G-protein Gq
Thrombin-activated PARs:Gq (active) dissociate
Thrombin-activated PARs:G12/13 (active) dissociate
Gbeta:gamma activation of PLC beta
PLC beta-mediated PIP2 hydrolysis
Dissociation of the P2Y purinoceptor 1:Gq complex
Opsins act as GEFs for G alpha-t
Activated TP receptor binds G-proten Gq
Gq activation by TP receptor
Dissociation of the TP:Gq complex
Activated TP receptor binds G-protein G13
G13 activation by TP receptor
Dissociation of the TP:G13 complex
Activation of GRIK3 homomer
TRiC/CCT binds unfolded G-protein beta subunit
ATP hydrolysis promotes folding of G-protein beta by TRiC/CCT
PDCL binds G-protein beta and TRiC/CCT
ATP binds G-protein beta associated TRiC/CCT
PDCL promotes formation of G-beta:G-gamma heterodimers
The Ligand:GPCR:Gs complex dissociates
Liganded Gs-activating GPCRs bind inactive heterotrimeric Gs
Liganded Gz-activating GPCRs bind inactive heterotrimeric G-protein Gz
Liganded Gq-activating GPCRs bind inactive heterotrimeric Gq
The Ligand:GPCR:Gq complex dissociates
Liganded Gz-activating GPCR acts as a GEF for Gz
The Ligand:GPCR:Gi complex dissociates
Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
Inactive G alpha (z) reassociates with G beta:gamma
Inactive G alpha (q) reassociates with G beta:gamma
Inactive G alpha (i) reassociates with G beta:gamma
Inactive G alpha (s) reassociates with G beta:gamma
The Ligand:GPCR:G12/13 complex dissociates
The Ligand:GPCR:Gz complex dissociates
Liganded G12/13-activating GPCRs bind inactive heterotrimeric G-protein G12/13
Liganded G12/13-activating GPCR acts as a GEF for G12/13
Inactive G alpha (12/13) reassociates with G beta:gamma
Glucagon:GCGR mediates GTP-GDP exchange
PDCL releases folded G-beta from TRiC/CCT
G-protein alpha releases G-protein heterotrimer from PDCL
G beta-gamma complex and PIP3 recruit BTK to membrane
BTK in PIP3:BTK:G beta-gamma complex is activated
PIP3:BTK:G beta-gamma complex dissociates to Active BTK, PIP3 and G beta-gamma complex
Active PAK1 and Active CDC42 dissociates from GNB/GNG:PAK1:ARHGEF6:CDC42 complex
CDC42 in GNB/GNG:PAK1:ARHGEF6:CDC42 is activated
PAK1:ARHGEF6 complex binds G-protein beta-gamma complex
PAK1 in GNB/GNG:PAK1:ARHGEF6:Active CDC42 is activated
CDC42 binds GNB/GNG:PAK1:ARHGEF6 complex
G-alpha(t):G-beta-gamma:Opsins complex dissociates to active G-alpha(t)
G-proteins dissociate from plasma-membrane estrogen receptors
Heterotrimeric G protein (i) binds membrane-associated ESRs:ESTG
ESTG stimulates GTP exchange on GPER:heterotrimeric G-protein s
GPER:Heterotrimeric G-protein Gs binds ESTG
ESTG-stimulated dissociation of GPER:heterotrimeric G-protein (s) complex
ADORA2B:Ade-Rib:GsGTP dissociates
ADORA2B (in Ade-Rib:ADORA2B:Heterotrimeric G-protein Gs) exchanges GDP for GTP on Gs
ADORA2B:Ade-Rib binds heterometric G-protein Gs
odorant binds Odorant Receptor:GNAL:GDP:GNB1:GNGT1
L-Glutamate or L-Aspartate and 5' nucleotide bind umami taste receptor (TAS1R1:TAS1R3)
Sweet compound binds sweet taste receptor (TAS1R2:TAS1R3)
Bitter compound binds bitter taste receptor (TAS2R)
The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma 2.
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Gbeta is a seven-bladed WD40 propeller with an extended Ggamma interface.
"Repeated WD motifs in beta form a circularized sevenfold beta propeller."
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Gbeta contacts nearly all of Ggamma.
"an extended interface between beta and nearly all of gamma"
Structures of the Rhodopsin-Transducin Complex: Insights into G-Protein Activation.
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Nucleotide exchange occurs on the Galpha subunit of transducin.
"activates the G-protein transducin (GT) by catalyzing GDP-GTP exchange on its α subunit (GαT)"
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The GalphaT helical domain contacts Gbetagamma in receptor-bound structures.
"capture three distinct conformers showing the GαT helical domain (αHD) contacting the Gβγ subunits"
Stimulation of phospholipase C by guanine-nucleotide-binding protein beta gamma subunits.
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Transducin betagamma stimulates PLC-beta.
"beta gamma subunits, purified from bovine retinal transducin (beta gamma t), markedly stimulated the hydrolysis of phosphatidylinositol 4,5-bisphosphate by this phospholipase C preparation"
Functional reconstitution of G-protein-coupled receptor-mediated adenylyl cyclase activation by a baculoviral co-display system.
Germline De Novo Mutations in GNB1 Cause Severe Neurodevelopmental Disability, Hypotonia, and Seizures.
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De novo GNB1 variants disrupt Galpha-Gbetagamma or effector interfaces in neurodevelopmental disease.
"The majority of these 13 mutations affect known Gβ binding sites, which suggests that a likely disease mechanism is through the disruption of the protein interface required for Gα-Gβγ interaction"
Mutations in G protein β subunits promote transformation and kinase inhibitor resistance.
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Somatic GNB1 mutations disrupt the Galpha interface and activate signaling.
"Multiple mutations in GNB1 affect the protein interface that binds Gα subunits as well as downstream effectors and disrupt Gα interactions with the Gβγ dimer."
Ligand-dependent G protein dynamics underlying opioid efficacy.
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The study used human Gbeta1 with Ggamma2.
"we used a single virus containing the genes for both the human Gβ1 and Gγ2 subunits"
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The open Galphai AHD may be affixed against Gbeta.
"possibly because the AHD is affixed against Gβ, as seen in a recent cryo-EM structure14"