Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Combined Automated Annotation using Multiple IEA Methods
The transcriptional regulator Np20 is the zinc uptake regulator in Pseudomonas aeruginosa.
-
Np20 (PA5499) is the P. aeruginosa Zur; it is zinc-responsive and represses both znuA and the zur-znuC-znuB operon, so the zinc-sensing step of this system belongs to the regulator, not to the transporter subunits.
"ZurPA is zinc responsive and represses transcription of both znuA and the zur-znuC-znuB operon in a zinc dependent manner."
-
Deletion of znuA, znuB or znuC reduces growth in LB containing 0.5 mM EDTA, with no general growth defect in plain LB; this is the evidence behind the PseudoCAP annotation on znuC.
"the inability to express the putative znuA, znuB, or znuC genes resulted in decreased growth at 18 hours as compared wild-type strain PAO1 when the strains were grown in LB media supplemented with 0.5 mM EDTA"
The capability of Pseudomonas aeruginosa to recruit zinc under conditions of limited metal availability is affected by inactivation of the ZnuABC transporter.
-
A znuA mutant of P. aeruginosa PA14 has only a limited growth defect in zinc-depleted media and near-normal intracellular zinc, indicating redundant zinc acquisition routes, but it shows reduced alginate production, lower activity of secreted zinc-dependent proteases and impaired dissemination in systemic infection.
"These findings suggest that P. aeruginosa is equipped with redundant mechanisms for the acquisition of zinc that might favor P. aeruginosa colonization of environments containing low levels of this metal."
UniProt entry Q9HT73 (ZNUC_PSEAE)
znuC bioinformatics - PAINT node PTN001952271 composition and propagation