NOV1 is a resveratrol-cleaving stilbene cleavage oxygenase with a seven-bladed beta-propeller and a four-His non-heme iron center; solved with resveratrol (substrate) and vanillin (product).

Catalysis requires deprotonation of the substrate 4-hydroxyl to activate it for the ferric- superoxide, so NOV1 cleaves a wide range of 4'-OH stilbene-like compounds.

NOV1 also cleaves the lignin-derived phenylpropene isoeugenol to vanillin and has been engineered for improved conversion - a lignin-valorization application.