Early evolution of the T-box transcription factor family.
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CoBra partially rescues dominant-negative XBra Xenopus embryos.
"Our data show that C. owczarzaki Brachyury ( CoBra ) can partially rescue Xenopus laevis embryos injected with a dominant negative XBra construct."
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Unlike animal Brachyury, CoBra activates all mesendodermal genes tested in Xenopus.
"which strongly activated all mesendodermal genes"
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Chimeras map the specificity difference to the N- and C-terminal regions, not the T-box.
"N-terminal and C-terminal domains, even though they do not contain any recognizable conserved amino acidic motifs, could largely account for the metazoan Brachyury homologs specificity"
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CoBra binds a DNA motif highly similar to that of mouse Brachyury (PBM).
"Our results indicate that CoBra has a highly similar motif to that determined in the mouse Bra-homolog, called T"
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CoBra has Arg at the position of Xenopus Bra Lys149.
"despite the presence of an Arginine (R) instead of a Lysine (K) in the CoBra protein"