Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
An endogenous protein inhibitor, YjhX (TopAI), for topoisomerase I from Escherichia coli.
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YjhQ is the antitoxin component of the topAI-yjhQ toxin-antitoxin system. TopAI (YjhX) inhibits topoisomerase I, causing cell death. YjhQ co-expression neutralizes TopAI toxicity. YjhQ physically interacts with TopAI in pull-down assays.
"we found a novel, previously unidentified TA system in Escherichia coli named yjhX-yjhQ. Induction of YjhX (85 amino acid residues) causes cell-growth arrest resulting in cell death, while YjhQ (181 residues) co-induction resumes cell growth"
YdgG (TqsA) Controls Biofilm Formation in<i>Escherichia coli</i>K-12 through Autoinducer 2 Transport
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yjhQ (b4307) shows biofilm-associated upregulation with an expression ratio of 11.3 in transcriptomics data. Listed as a hypothetical ORF of 181 aa.
"yjhQ/b4307 listed in table of genes with altered expression, categorized under Transport, with expression ratio 11.3"
Phosphoproteome study of Escherichia coli devoid of Ser/Thr kinase YeaG during the metabolic shift from glucose to malate.
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YjhQ is phosphorylated at Thr11 in vivo, and phosphorylation occupancy is significantly reduced in a delta-yeaG kinase mutant during glucose-to-malate shift. Suggests YjhQ participates in a kinase-regulated response to carbon-source transition.
"phosphorylation of YjhQ at Thr11 is significantly reduced in delta-yeaG versus wild type during glucose to malate shift"
Identification of novel protein lysine acetyltransferases in Escherichia coli.
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yjhQ is present in the ASKA overexpression library (JW4269, pCA24n-yjhQ) and was included as a candidate in a systematic E. coli lysine acetyltransferase discovery screen, though no positive functional result was reported for YjhQ.
"JW4269 is listed as ASKA pCA24n-yjhQ in strain/plasmid resources for the acetyltransferase study"
Limitations of current machine learning models in predicting enzymatic functions for uncharacterized proteins.
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DeepECTF predicted EC 2.3.1.189 (mycothiol synthase) for YjhQ, which is biologically impossible because mycothiol biosynthesis is entirely absent from E. coli. This represents a Non-Paralog Incorrect (NPI) prediction error category.
"YjhQ/b4307 is predicted to be a mycothiol synthase (EC 2.3.1.189), but mycothiol is not a molecule synthesized by E. coli, and the remaining pathway genes are absent from the genome (BioCyc ID: PWY1G-0)"