PAS2 (PASTICCINO2/PEPINO; At5g10480; Q8VZB2) review notes

Identity and function

ER 3-hydroxyacyl-CoA dehydratase of the VLCFA elongase, PHS1/HACD ortholog PMID:18799749.
Reciprocal complementation with yeast PHS1; pas2-1 accumulates 3-hydroxyacyl-CoAs PMID:18799749.
ER localization; BiFC with CER10/ECR PMID:18799749.
Null alleles embryo lethal; pas2-1 has reduced VLCFAs in TAG, wax, sphingolipids.
Historical PTP-like classification: mutated PTP site, no phosphatase activity PMID:16698944; dehydratase catalytic residues are not in the PTP motif PMID:18799749.
CDKA;1 "antiphosphatase" model and cytosolic/nuclear localization come from overexpressed 35S-PAS2:GFP PMID:16698944; later PHS1 complementation shows developmental defects are due to defective dehydratase PMID:18799749.
Deep research (file:ARATH/PAS2/PAS2-deep-research-falcon.md) also mentions a Golgi pool (Zhu et al. 2020) linked to cellulose synthase complexes - not in GOA, not reviewed further (paper not cached).

REMOVE: protein tyrosine phosphatase activity (ISS; contradicted), protein binding (IPI), mitochondrion (ISM).
UNDECIDED: nucleus (IDA and IEA) - single overexpression GFP study vs integral ER membrane protein; needs native-promoter validation.
MARK_AS_OVER_ANNOTATED: cytosol (IDA), negative regulation of developmental growth (IMP, overexpression phenotype).
KEEP_AS_NON_CORE: cell differentiation (IMP), regulation of cell division (IMP).
ACCEPT: dehydratase activities, elongase complex (IPI), ER/ER membrane, cytoplasm (general), FA elongation, VLCFA biosynthesis, sphingolipid biosynthesis (IBA), FA biosynthesis (IEA).

Supersedes the "UNDECIDED: nucleus (IDA and IEA)" line above.

GO:0005634 nucleus IDA (PMID:16698944) and the matching IEA (UniProt subcellular location derived from the same study) are both MARK_AS_OVER_ANNOTATED. The cached full text shows the nuclear signal came from a 35S-driven C-terminal GFP fusion in lines selected for high GFP PMID:16698944, with perinuclear accumulation in dividing cells consistent with nuclear envelope/ER. PAS2 is a multi-pass ER-membrane 3-hydroxyacyl-CoA dehydratase; ER membrane is the supported location. Not removed, because nuclear GFP signal was genuinely recorded.