Ces2c review notes

Evidence summary

• PMID:12230550 The publication supports Ces2c retinyl ester hydrolase/retinol metabolism annotations.
• [UniProtKB:O70631] UniProt summarizes Ces2c as a hydrolase with high activity toward palmitoylcarnitine and possible retinyl ester hydrolysis.

Curation decisions

• Core function: acylcarnitine/retinyl ester hydrolase (acylcarnitine hydrolase activity, GO:0047619).
• Specific catalytic activities were accepted; broad parent terms were modified to the specific activity where possible.
• Localization, cofactor/binding, and phenotype-level annotations were retained only as non-core unless directly tied to the enzymatic role.

Re-review 2026-10-04

• GOA refresh (commit a3cf70b6d): no new rows and no retired rows; WITH/FROM and qualifiers
  backfilled on the 13 existing rows. 0 PENDING.
• Description rewritten as standalone biology (the previous text described what "the review
  keeps"). New text uses UniProt O70631: acylcarnitine hydrolase (EC 3.1.1.28), retinyl
  hexadecanoate -> all-trans-retinol (RHEA:13933), "Microsome {ECO:0000269|PubMed:12230550}",
  expressed in liver, stomach and kidney, signal peptide 1..26.
• GO:0043231 intracellular membrane-bounded organelle (IDA, PMID:12230550):
  MARK_AS_OVER_ANNOTATED -> MODIFY to GO:0005783 endoplasmic reticulum. The problem is
  generality, not an overreaching claim; the experiment purified the enzyme "from rat liver
  microsomal extracts" PMID:12230550, and microsomes are ER-derived (GO has no current
  microsome CC term).
• GO:0050253 retinyl-palmitate esterase activity (IDA, PMID:12230550) kept ACCEPT with added
  provenance. The cached abstract (abstract-only) lists AB010635 (the Ces2c EMBL entry) among
  the purified isoenzymes but explicitly names only "D50580 and AY034877 also hydrolyzed retinyl
  palmitate"; the full text is not cached, so the curator/UniProt reading is deferred to.
• Open question: confirm from the full text of PMID:12230550 the retinyl palmitate hydrolase
  rate measured for the AB010635 (Ces2c) protein.

Stale UniProt quotes (2026-10-10): replaced 6 UniProtKB:O70631 supporting_text quotes (legacy DR GO; lines with evidence codes no longer present in the refreshed flat file) with verbatim SUBCELLULAR LOCATION, CATALYTIC ACTIVITY (acylcarnitine hydrolysis) and FUNCTION text from the current Ces2c-uniprot.txt.

Review follow-up 2026-10-17

Addressed PR #4615 reviewer suggestion: removed blank lines inside quoted YAML scalars (7)
that were leaking hard newlines into the rendered HTML. The self-referential DR GO;
supporting_text quotes were left as-is (see cross-gene note). No action changed. Revalidated
and re-rendered.