Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Cap-specific terminal N (6)-methylation of RNA by an RNA polymerase II-associated methyltransferase.
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PCIF1/CAPAM is the cap-specific adenosine methyltransferase responsible for N6-methylation of m6Am at the transcription start nucleotide.
"as a cap-specific adenosine methyltransferase (CAPAM) responsible for N 6-methylation of m6Am"
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Crystal structures of CAPAM (the zebrafish construct, per UniProt) with SAM and capped RNA defined the basis of cap-specific m6A formation.
"The crystal structure of CAPAM in complex with substrates revealed the molecular basis of cap-specific m6A formation."
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PCIF1 interacts with the Ser5-phosphorylated RNA polymerase II CTD.
"interacts with the serine-5-phosphorylated carboxyl-terminal domain of RNA polymerase II"
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The paper reports that m6Am promotes translation of capped mRNAs; this conclusion is contested by later studies.
"A transcriptome-wide analysis revealed that N 6-methylation of m6Am promotes the translation of capped mRNAs."
UniProtKB entry A0A0R4IKJ1 for Danio rerio pcif1
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UniProt describes zebrafish Pcif1 as the cap-specific adenosine methyltransferase forming the m6Am cap.
"Cap-specific adenosine methyltransferase that catalyzes"
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The zebrafish protein was crystallized with SAM and m7G-capped RNA and assayed for catalytic activity and mutagenesis.
"S-ADENOSYL-L-METHIONINE AND M7G-CAPPED RNA, FUNCTION, CATALYTIC ACTIVITY,"
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Nuclear localization is a by-similarity statement from human PCIF1.
"SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9H4Z3}"
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The effect of m6Am on translation is unresolved.
"transcripts is unclear and subject to discussion."
Falcon (Edison) deep research report on Danio rerio pcif1/CAPAM
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The report identifies the zebrafish CAPAM crystal structures and detection of m6Am in zebrafish mRNA as the only zebrafish-specific evidence, and notes the absence of zebrafish genetic data.
"m6Am is detectable in zebrafish mRNA"
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The report notes that CAPAM acts only at the cap-adjacent position, not on internal Am.
"CAPAM does **not methylate internal Am**"