Msed_0639 (A4YEG2) notes — methylmalonyl-CoA epimerase
Automated deep research was unavailable for this gene (no *-deep-research-*.md
file). The literature below was located manually (Europe PMC / NCBI E-utilities /
web search) and the cited PMIDs are cached in publications/.
Identity
- UniProt A4YEG2 (unreviewed), 140 aa, "Methylmalonyl-CoA epimerase", EC 5.1.99.1.
VOC (vicinal oxygen chelate) fold: PROSITE PS51819, InterPro IPR017515
(MeMalonyl-CoA_epimerase), NCBIfam TIGR03081 (metmalonyl_epim), CDD cd07249 (MMCE),
PANTHER PTHR43048:SF3 [file:METS5/Msed_0639/Msed_0639-uniprot.txt "Methylmalonyl-CoA epimerase"].
- Gene is adjacent to Msed_0638 (MCM catalytic subunit).
Literature
Conclusions
- Msed_0639 is the recombinantly verified homodimeric, divalent-cation-activated
methylmalonyl-CoA epimerase of the 3HP/4HB cycle, converting the (S)-methylmalonyl-CoA
made by propionyl-CoA carboxylase to (R)-methylmalonyl-CoA for MCM; transcription is
induced under autotrophy. No genetic (knockout) evidence exists.
- GO:0004493 accepted; GO:0046491 L-methylmalonyl-CoA metabolic process kept as non-core (the
enzyme interconverts the two methylmalonyl-CoA epimers, so L-methylmalonyl-CoA is its
substrate or product in either direction; note the GO definition itself mixes 'L-' and
'S-', whereas L-methylmalonyl-CoA is conventionally the (R)-epimer used by MCM); GO:0015977 carbon fixation NEW.
- Metal activation (Ni, Co, Mg) is consistent with the VOC fold but the bound metal in
vivo is not known; no metal-binding term is proposed.