Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods
Zebrafish hairy/enhancer of split protein links FGF signaling to cyclic gene expression in the periodic segmentation of somites.
-
The primary reporter and interaction experiments establish transcriptional repression and Her1 association for Her13.2/Hes6.
"Her13.2 augments autorepression of her1 in association with Her1 protein."
-
Mammalian Hes6 is the only structural counterpart of her13.2, and Her13.2 needs a bHLH partner because of its short HLH loop.
"Her13.2 has a uniquely short loop region within its HLH domain, causing an inability to bind to the target sequences"
-
Primary zebrafish perturbation links Hes6 to the segmentation clock and somite formation.
"her13.2 is required for periodic repression of the
Notch-regulated genes her1 and her7, and for proper somite segmentation."
her1 and her13.2 are jointly required for somitic border specification along the entire axis of the fish embryo.
Segment number and axial identity in a segmentation clock period mutant.
-
The period-mutant study supports clock-dependent segment number; target identity is independently corroborated by the full 2018 report.
"precise control of segmentation
clock period in relation to axial growth ensures a species-specific segment
number"
-
The period-mutant study distinguishes segment number from Hox-associated regional identity.
"arguing
against an instructive role of the segmentation clock in determining axial
identities."
Topology and dynamics of the zebrafish segmentation clock core circuit.
-
Direct zebrafish biochemistry establishes partner-dependent DNA binding by Hes6 in the Her7:Hes6 heterodimer.
"only Her1 homodimers and Her7:Hes6 heterodimers have strong DNA binding activity and target similar DNA sites."
-
Hes6 forms heterodimers and homodimers; their DNA-binding capabilities differ.
"all of the possible dimers between Her1, Her7 and Hes6 form"
-
Zebrafish Hes6 protein is nuclear in the PSM, shown with a Hes6 monoclonal antibody whose signal is absent in hes6 mutants.
"Antibodies show immunoreactivity with a nuclear antigen in wildtype, but not in hes6 mutant embryos."
-
Hes6 is not cyclically transcribed but has an FGF-dependent graded expression; its protein levels oscillate post-transcriptionally through dimerization with Her1 and Her7.
"Although Hes6 production is constant in time, Hes6 levels oscillate due to rhythmic changes in effective stability."
-
MyoD association is experimentally measured but is used to demonstrate promiscuous bHLH interactions, not a physiological Hes6 myogenesis mechanism.
"even the bHLH containing factor MyoD, but not the negative control non-bHLH protein PPARĪ³, was co-purified by Her1, Her7, and Hes6 to a similar extent"
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock.
-
The full primary report directly generates and analyzes a hes6 mutant; its focus on notochord-derived centra does not make the Hes6 annotation a wrong-gene citation.
"The novel hes6 mutation was created by injection of a TALEN targeted against hes6 in wild type."
-
The full figure caption reports disordered myotome boundaries in her1;hes6 mutants and compares multiple segmentation-clock mutant combinations.
"her1;hes6 mutants (n=15)"
Hes6 acts in a positive feedback loop with the neurogenins to promote neuronal differentiation.
-
Hes6 from the HES6 subfamily (mouse/Xenopus) is expressed in developing neurons and promotes neurogenesis on ectopic expression, the opposite sign to classical HES/E(spl) inhibitors.
"When ectopically expressed in Xenopus embryos, Hes6 promotes neurogenesis."
PTHR10985:SF11 membership and pairwise identity of zebrafish hes6 and HES6 orthologs
UniProt identities of the three experimental GOA interaction partners
Frozen exact-input ProtNLM output and UniProt sequence for Q6P0J1
Falcon deep research report on Danio rerio hes6 (Q6P0J1)