GO_REF:0000015

Use of the ND evidence code for Gene Ontology (GO) terms

GO_REF:0000033

Annotation inferences using phylogenetic trees

The ubiquitin ligase complex annotation on MOH1 is a phylogenetically propagated (IBA) inference from PANTHER family PTHR13848, seeded by human YPEL5 (UniProtKB:P62699); there is no yeast-specific experimental support.

PMID:30358795

The cellular economy of the Saccharomyces cerevisiae zinc proteome.

MOH1 is included among 582 known or potential zinc-binding proteins identified by a proteome-wide bioinformatic domain/motif survey; this is the basis of the RCA zinc ion binding annotation, a prediction rather than a direct metal-binding assay of Moh1.

PMID:28173693

Pro-Apoptotic Role of the Human YPEL5 Gene Identified by Functional Complementation of a Yeast moh1Δ Mutation.

moh1 deletion increases cell viability under multiple lethal stresses (UV, MMS, camptothecin, heat, hyperosmotic shock), and Moh1 is stress-induced; human YPEL genes partially complement moh1delta. Framed by the authors as involvement in DNA-damage-induced, mitochondria-dependent regulated cell death.

PMID:9748261

A role for Saccharomyces cerevisiae fatty acid activation protein 4 in regulating protein N-myristoylation during entry into stationary phase.

YBL049W (MOH1) was identified as one of nine putative N-myristoylproteins whose deletion reproduces the stationary-phase survival defect of nmt1 mutant cells, linking MOH1 phenotypically to maintenance of proliferative potential during stationary phase.

PMID:11240639

The Drosophila gene Yippee reveals a novel family of putative zinc binding proteins highly conserved among eukaryotes.

Defines the Yippee family as a highly conserved eukaryotic family bearing a putative zinc-finger-like metal-binding domain; even at the family's founding the molecular function was left open.

PMID:15456898

Genomic analysis of stationary-phase and exit in Saccharomyces cerevisiae: gene expression and identification of novel essential genes.

Genome-wide screen that identified MOH1 as one of the stationary-phase-essential genes: moh1 deletion causes a large loss of viability during long-term stationary phase at 37C, yet MOH1 is not required for growth on nonfermentable carbon sources, indicating a specific quiescence-survival role rather than a generic respiratory defect.

file:yeast/MOH1/MOH1-deep-research-falcon.md

Falcon deep research report on S. cerevisiae MOH1 (YBL049W)

Synthesis concluding MOH1 is a small conserved Yippee-family regulatory/adaptor protein with a zinc-stabilized beta-tent fold and cradle-shaped binding pocket, required for stationary-phase survival, whose precise biochemical activity, substrate, and dedicated pathway remain unresolved in the retrievable literature.

file:yeast/MOH1/MOH1-bioinformatics/RESULTS.md

Bioinformatics analysis of MOH1 (Yippee domain, zinc site, ortholog conservation)

Independently confirms the Yippee zinc signature (C45-x2-C48 ... C101-x2-C104, 52-residue spacer) matching the UniProt Zn-binding features, ~37-45% identity to human YPEL1-5 with all four Zn-cysteines perfectly conserved, and no sequence-level evidence for catalytic activity.