Gene Ontology annotation through association of InterPro records with GO terms

Source of the IEA GO:0051983 annotation, via InterPro IPR044951 (SPC24-like) to "regulation of chromosome segregation".

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

Basis of the ISS GO:0031262 (Ndc80 complex) annotation, transferred from human SPC24 (Q8NBT2).

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

Source of the IEA GO:0000775 (chromosome, centromeric region) annotation, from the UniProt centromere subcellular-location term.

GO_REF:0000122

AtSubP analysis

Source of the ISM GO:0005634 (nucleus) prediction.

PMID:29356153

MUN (MERISTEM UNSTRUCTURED), encoding a SPC24 homolog of NDC80 kinetochore complex, affects development through cell division in Arabidopsis thaliana.

SPC24 is a subunit of the four-protein NDC80 outer-kinetochore complex that connects spindle microtubules to the kinetochore and mediates chromosome segregation during cell division.

"The NDC80 complex, composed of four proteins, NDC80, NUF2, SPC24 and SPC25, is localized at the outer kinetochore and connects spindle fibers to the kinetochore."
AtSPC24/MUN colocalizes with the centromeric histone variant CENH3 at the centromere; interactions among NDC80-complex components were confirmed by yeast two-hybrid and in planta co-immunoprecipitation.

"Interactions among the components of the NDC80 complex were confirmed in a yeast two-hybrid assay and in planta co-immunoprecipitation."
mun mutants show embryo arrest, DNA aneuploidy, chromosome-segregation defects and a low cell-division rate; null alleles are embryonic lethal.

"The mutant exhibits stunted growth, embryo arrest, DNA aneuploidy, and defects in chromosome segregation with a low cell division rate."

PMID:33993566

AtNUF2 modulates spindle microtubule organization and chromosome segregation during mitosis.

The NDC80-complex subunit AtNUF2 modulates spindle microtubule organization and chromosome segregation during mitosis, corroborating the complex's mitotic role.