Gene Ontology annotation based on Enzyme Commission mapping
Combined Automated Annotation using Multiple IEA Methods
Characterization of TCL, a new GTPase of the rho family related to TC10 andCcdc42.
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Full-length human TCL/RHOJ undergoes rapid GDP/GTP exchange and has intrinsic GTP hydrolysis activity higher than that of TC10.
"In vitro, TCL shows rapid GDP/GTP
exchange and displays higher GTP dissociation and hydolysis rates than TC10."
TCL/RhoJ Plasma Membrane Localization and Nucleotide Exchange Is Coordinately Regulated by Amino Acids within the N Terminus and a Distal Loop Region.
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RHOJ nucleotide loading and plasma-membrane localization depend on N-terminal residues and on a distal loop (residues 121-129) that is absent from G3V4H1.
"Chimeras of TCL and TC10
revealed amino acids 121-129 of TCL contributed to the differences in nucleotide
loading."
RhoJ is an endothelial cell-restricted Rho GTPase that mediates vascular morphogenesis and is regulated by the transcription factor ERG.
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RHOJ is expressed in an endothelial-restricted pattern, and its knockdown blocks endothelial lumen and tube formation and in vivo angiogenesis in Matrigel plugs.
"knock-down of RhoJ blocked lumen formation significantly in 3D collagen matrices"
Role of glutamine synthetase in angiogenesis beyond glutamine synthesis.
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In endothelial cells, glutamine synthetase (GLUL) interacts with RHOJ and sustains its palmitoylation, membrane localization and activation, which are needed for endothelial migration.
"glutamine synthetase palmitoylates itself and interacts with RHOJ to sustain RHOJ palmitoylation, membrane localization and activation."
Exact RHOJ product-to-reference sequence mapping
RHOJ G3V4H1 re-review: product identity and G-domain completeness
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G3V4H1 is translated from the NMD-biotype transcript ENST00000557447. It shares residues 1-103 with Q9H4E5 and then reads a 50-residue tail from the canonical 3' UTR, losing the G4, G5 and CAAX motifs.
"G3V4H1 is therefore not either of the two UniProt-described isoforms of Q9H4E5"
Frozen exact-input ProtNLM output and UniProt sequence for G3V4H1