Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Combined Automated Annotation using Multiple IEA Methods
Novel conserved assembly factor of the bacterial flagellum.
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FliW (yviF) interacts with flagellin and is required for motility
"TP0658 (FliW) and its orthologs, conserved proteins of unknown function in Treponema pallidum and other species, interact with a C-terminal region of flagellin"
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fliW mutants show strongly reduced swarming motility
"Mutants of orthologs in Bacillus subtilis and Campylobacter jejuni (yviF, CJ1075) showed strongly reduced motility"
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FliW stabilizes flagellin similar to FliS, suggesting a role as assembly factor
"TP0658 stabilizes flagellin in a way similar to FliS, suggesting that TP0658 is a conserved assembly factor for the bacterial flagellum"
CsrA-FliW interaction governs flagellin homeostasis and a checkpoint on flagellar morphogenesis in Bacillus subtilis.
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FliW is the first identified protein antagonist of CsrA activity
"Here we discover FliW, the first protein antagonist of CsrA activity that constitutes a partner switching mechanism to control flagellin synthesis"
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FliW binds CsrA and relieves CsrA-mediated translational repression of hag
"FliW then binds to CsrA and relieves CsrA-mediated translational repression of hag for flagellin synthesis concurrent with filament assembly"
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Partner switching between FliW-Hag and FliW-CsrA complexes provides a checkpoint for flagellin synthesis
"secretion of flagellin (Hag) releases FliW protein from a FliW-Hag complex"
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Flagellin homeostatically restricts its own translation
"Thus, flagellin homeostatically restricts its own translation"
FliW antagonizes CsrA RNA binding by a noncompetitive allosteric mechanism.
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FliW binds CsrA at an allosteric site distinct from the RNA-binding site
"FliW of Bacillus subtilis does not bind to the same residues of CsrA required for RNA binding"
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FliW noncompetitively inhibits CsrA binding to hag RNA
"We conclude that FliW inhibits CsrA by a noncompetitive mechanism that differs dramatically from the well-established sRNA inhibitors"
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Two FliW molecules bind per CsrA dimer
"residues of CsrA on an allosteric surface of previously unattributed function"
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FliW has a highly negatively charged surface that provides electrostatic repulsion against RNA
"FliW and RNA interaction is not mutually exclusive"
UniProtKB entry for FliW (P96503)
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FliW acts as an anti-CsrA protein
"Acts as an anti-CsrA protein, binds CsrA and prevents it from repressing translation of its target genes, one of which is flagellin"
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FliW binds CsrA at an allosteric site
"An antagonist to translational regulator CsrA, it binds CsrA at an allosteric site and non-competitively inhibits CsrA binding to hag RNA"
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Partner switching controls flagellin synthesis timing
"Partner switching by flagellin between FliW and CsrA provides a flagellar assembly checkpoint to tightly control the timing of flagellin synthesis"
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fliW mutants have reduced motility and fewer flagella
"Greatly reduced swarming motility, less flagellin"
Deep research on fliW function