Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Combined Automated Annotation using Multiple IEA Methods
Isolation and cDNA cloning of a novel galanin-like peptide (GALP) from porcine hypothalamus.
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Isolated the 60-residue galanin-like peptide (GALP) from hypothalamus, cloned porcine, rat, and human GALP cDNAs, showed that GALP-(9-21) is 100% identical to galanin-(1-13), and demonstrated that GALP is a high-affinity endogenous agonist for GALR2 (IC50 = 0.24 nM) and GALR1 (IC50 = 4.3 nM) that stimulates [35S]GTPgammaS binding.
"We isolated a novel galanin-like peptide (GALP) from porcine hypothalamus by observing its activity for increasing [(35)S]GTPgammaS binding to a membrane preparation of GALR2-transfected cells. The peptide had 60 amino acid residues and a non-amidated C terminus. The amino acid sequence of GALP-(9-21) was completely identical to that of galanin-(1-13)."
A reference map of the human binary protein interactome.