Gene Ontology annotation through association of InterPro records with GO terms
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry Q88FA4 for Pseudomonas putida KT2440 gltA
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UniProt records EC 2.3.3.16 for the Q88FA4 citrate synthase reaction.
"EC=2.3.3.16;"
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UniProt records the oxaloacetate/acetyl-CoA citrate synthase reaction.
"Reaction=oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+);"
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UniProt places gltA in the TCA cycle.
"from oxaloacetate: step 1/2."
OpenScientist deep research report for gltA
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The report supports GltA as the cytoplasmic citrate synthase at the acetyl-CoA entry point to the TCA cycle.
"Functionally, GltA operates in the **cytoplasm** and serves as the **gatekeeper of the acetyl-CoA node**"
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The report assigns Q88FA4 to the Gram-negative Type II citrate synthase class.
"This places Q88FA4 unambiguously in the **Gram-negative "Type II"** class of citrate synthases"
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The report characterizes Gram-negative Type II citrate synthases as NADH-regulated hexameric enzymes.
"hexameric enzymes that are **allosterically inhibited by NADH**"
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Direct Q88FA4 enzymology is absent, so kinetic and NADH-inhibition parameters remain transferred from homologs.
"No direct enzymology on Q88FA4 itself."
Model-guided dynamic control of essential metabolic nodes boosts acetyl-coenzyme A-dependent bioproduction in rewired Pseudomonas putida.
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Kozaeva et al. identify P. putida gltA as encoding citrate synthase.
"gltA, encoding citrate synthase"
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CRISPRi-mediated reduction of gltA and accA expression raised acetyl-CoA in rewired P. putida.
"encoding subunit A of the acetyl-CoA carboxylase complex) mediated an 8-fold
increase in the acetyl-CoA content of rewired P. putida."
Cloning, sequencing, and expression of the gene for NADH-sensitive citrate synthase of Pseudomonas aeruginosa.
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The P. aeruginosa allosteric citrate synthase gene probe detected highly homologous sequences in P. putida genomic DNA.
"A portion of the cloned P.
aeruginosa gene was used as a hybridization probe under stringent conditions to
identify highly homologous sequences in genomic DNA of a second strain
classified as P. aeruginosa and isolates of P. putida, P. stutzeri, and P.
alcaligenes."
Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440