Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
RGD ISO annotations to rat from other mammalian species
Enzymic changes in the cervix of the rat and hamster during the oestrous cycle and the effect of steroids.
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Estradiol induces cervical arylsulphatase B activity in ovariectomised rats; cycloheximide blocks the induction, indicating regulation at the level of protein biosynthesis
"Cycloheximide prevented the rise in arylsulphatase B activity occurring after oestrogen injection, suggesting a regulation of"
Inability of thiol compounds to restore CNS arylsulfatases inhibited by methyl mercury.
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Methylmercury dose-dependently inhibits rat CNS arylsulfatase activity; thiol antagonists (NAHT, GSH) do not restore activity
"a dose and duration dependent"
Cell-surface arylsulfatase A and B on sinusoidal endothelial cells, hepatocytes, and Kupffer cells in mammalian livers.
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Mammalian ARSA and ARSB localise to the cell surface of sinusoidal endothelial cells, hepatocytes, and Kupffer cells in liver, in addition to their lysosomal localisation
"mammalian ARSA and ARSB exist on the cell surface of sinusoidal endothelial cells, hepatocytes, and sinusoidal macrophages (Kupffer cells), as well as in the lysosome. They are also colocalized with heparan sulfate proteoglycan."
Characterization of liver lysosomal enzyme activity in hepatocytes, Kupffer and endothelial cells during aging: effect of dietary restriction.
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Rat hepatic arylsulfatase B activity increases with age and is further elevated by dietary restriction in Kupffer/endothelial cells
"Rats exposed to dietary restriction (R) showed higher activities of beta-galactosidase, arylsulfatase B and cathepsin D when compared to corresponding A animals with the exception of the younger age group."
Arylsulfatase B modulates neurite outgrowth via astrocyte chondroitin-4-sulfate: dysregulation by ethanol.
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Astrocyte Arsb silencing (or ethanol-mediated Arsb inhibition) increases sulfated GAGs, C4S, and neurocan and reduces cocultured hippocampal neurite outgrowth; recombinant Arsb rescues the ethanol effect
"ARSB silencing increased the levels of sulfated GAGs, C4S, and neurocan in astrocytes and inhibited neurite outgrowth in cocultured neurons, indicating that ARSB activity directly regulates C4S and affects neurocan expression."
Arylsulfatase B regulates versican expression by galectin-3 and AP-1 mediated transcriptional effects.
Isolation and characterization of rat hepatic ascorbic acid-2-sulfatases.
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Rat arylsulfatase B hydrolyses ascorbic acid-2-sulfate as a minor substrate (0.6% relative to p-nitrocatechol sulfate)
"The comparative biochemical properties suggest that arylsulfatase B is responsible for the cationic ascorbic acid-2-sulfatase activity"
The effect of pH on the kinetics of arylsulphatases A and B.
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Rat liver arylsulphatases A and B show pH-dependent activity with pK values of 4.4-4.5 and 5.7-5.8; a general acid-base-catalysed mechanism is proposed
"The effect of pH on the kinetics of rat liver arylsulphatases A and B is very similar and shows that two groups with pK values of 4.4-4.5 and 5.7-5.8 are important for enzyme activity"
Autophagy-related changes of arylsulphatases A and B in rat liver lysosomes.
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Arylsulphatase A/B activity ratio in rat liver lysosomes declines during enhanced autophagocytosis; A is converted to B by other lysosomal hydrolases active in autophagic vacuoles
"the arylsulphatase A/arylsulphatase B activity ratio in liver lysosomes of normal rats is maintained within rather narrow limits, and this ratio declines during enhanced autophagocytosis. These findings, together with observations that suggest that arylsulphatase B may be a partially degraded form of arylsulphatase A, are consistent with the view that the A form is more rapidly converted into the B form during autophagy, owing to the digestive activity of the other lysosomal hydrolases present in autophagic vacuoles."
Mucopolysaccharidosis type VI in rats: isolation of cDNAs encoding arylsulfatase B, chromosomal localization of the gene, and identification of the mutation.
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Rat Arsb cDNA is ~80/85% identical (nt/aa) to human ARSB; a homozygous 507insC frameshift mutation in the MPR rat colony causes rat MPS VI, an authentic model of human MPS VI
"The nucleotide and deduced amino acid sequences of rat ARSB had approximately 80 and 85% identity with the human ARSB sequences, respectively"
UniProt entry for rat Arsb (P50430)
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UniProt records rat Arsb as removing 4-sulfate groups from chondroitin-4-sulfate and regulating its degradation.
"FUNCTION: Removes sulfate groups from chondroitin-4-sulfate (C4S) and regulates its degradation (PubMed:24311516)."
Reviewer notes for rat Arsb