Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic Gene Ontology annotation based on Rhea mapping
Combined Automated Annotation using Multiple IEA Methods
Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways.
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ASK1 was identified as a MAP kinase kinase kinase (MAPKKK) that activates SEK1/MKK4 and MKK3/MKK6
"A MAP kinase kinase kinase (MAPKKK), termed ASK1, was identified that activated two different subgroups of MAP kinase kinases (MAPKK), SEK1 (or MKK4) and MKK3/MAPKK6 (or MKK6)"
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Overexpression of ASK1 induces apoptotic cell death
"Overexpression of ASK1 induced apoptotic cell death"
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ASK1 is activated by TNF-alpha
"ASK1 was activated in cells treated with tumor necrosis factor-alpha (TNF-alpha)"
Glutamine-dependent antiapoptotic interaction of human glutaminyl-tRNA synthetase with apoptosis signal-regulating kinase 1.
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ASK1 is activated during glutamine deprivation
"Fas ligation activated apoptosis signal-regulating kinase 1 (ASK1) and c-Jun N-terminal kinase (JNK; also known as stress-activated protein kinase (SAPK)) in Gln-deprived cells but not in normal cells"
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QRS interacts with ASK1 via catalytic domains to inhibit ASK1 activity
"The association involved the catalytic domains of the two enzymes. The ASK1 activity was inhibited by the interaction with QRS"
Akt phosphorylates and negatively regulates apoptosis signal-regulating kinase 1.
HIV-1 Nef inhibits ASK1-dependent death signalling providing a potential mechanism for protecting the infected host cell.
Apoptosis signal-regulating kinase 1 controls the proapoptotic function of death-associated protein (Daxx) in the cytoplasm.
Activation of apoptosis signal-regulating kinase 1 by the stress-induced activating phosphorylation of pre-formed oligomer.
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ASK1 forms homo-oligomers through C-terminal coiled-coil region
"ASK1 appears to form a silent homo-oligomer through its C-terminal coiled-coil region in non-stressed cells"
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Thr845 (Thr838 in human) phosphorylation is essential for activation
"phosphorylation of Thr845 at the activation loop is essential for ASK1 to be activated by H2O2"
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Oxidative stress triggers conformational change and autophosphorylation
"Following H2O2 treatment, pre-existing ASK1 oligomer undergoes conformational change and creates a new interface within an oligomer, which ultimately leads to trans-autophosphorylation of Thr845"
Scaffold role of a mitogen-activated protein kinase phosphatase, SKRP1, for the JNK signaling pathway.
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ASK1 interacts with SKRP1 (MKP)
"SKRP1 also interacted with the MAPKKK, apoptosis signal-regulating kinase 1 (ASK1)"
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SKRP1 enhances activation of MKK7 by ASK1
"SKRP1 expression increased the ASK1-MKK7 complexes in a dose-dependent manner and specifically enhanced the activation of MKK7 by ASK1"
ASK1 is essential for endoplasmic reticulum stress-induced neuronal cell death triggered by expanded polyglutamine repeats.
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ER stress activates ASK1 through IRE1-TRAF2-ASK1 complex formation
"ER stress activates ASK 1 through formation of an IRE1-TRAF2-ASK1 complex"
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ASK1-/- neurons are defective in ER stress-induced JNK activation and cell death
"ASK1(-/-) primary neurons are defective in polyQ-, proteasome inhibitor-, and ER stress-induced JNK activation and cell death"
Type 1 insulin-like growth factor receptor (IGF-IR) signaling inhibits apoptosis signal-regulating kinase 1 (ASK1).
AIP1 mediates TNF-alpha-induced ASK1 activation by facilitating dissociation of ASK1 from its inhibitor 14-3-3.
Identification of a novel antiapoptotic protein that antagonizes ASK1 and CAD activities.
Positive regulation of apoptosis signal-regulating kinase 1 by hD53L1.
Interaction of apoptosis signal-regulating kinase 1 with isoforms of 14-3-3 proteins.
S-nitrosation of thioredoxin in the nitrogen monoxide/superoxide system activates apoptosis signal-regulating kinase 1.
Interaction of DJ-1 with Daxx inhibits apoptosis signal-regulating kinase 1 activity and cell death.
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DJ-1 protects against oxidative stress by sequestering Daxx
"wild-type DJ-1 sequesters Daxx in the nucleus, prevents it from gaining access to the cytoplasm"
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Daxx activates ASK1 when in cytoplasm
"from binding to and activating its effector kinase apoptosis signal-regulating kinase 1"
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ASK1-/- primary neurons are defective in stress-induced cell death
"the regulated sequestration of Daxx in the nucleus and keeping apoptosis signal-regulating kinase 1 activation in check is a critical mechanism by which DJ-1 exerts its cytoprotective function"
Thioredoxin-ASK1 complex levels regulate ROS-mediated p38 MAPK pathway activity in livers of aged and long-lived Snell dwarf mice.
Human glutathione S-transferase P1-1 interacts with TRAF2 and regulates TRAF2-ASK1 signals.
Direct interaction and reciprocal regulation between ASK1 and calcineurin-NFAT control cardiomyocyte death and growth.
Apoptosis signal-regulating kinase (ASK) 2 functions as a mitogen-activated protein kinase kinase kinase in a heteromeric complex with ASK1.
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ASK1 forms heteromeric complex with ASK2
"ASK2, a highly related serine/threonine kinase to ASK1, also functions as a MAP3K only in a heteromeric complex with ASK1"
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ASK2 requires ASK1 for stability and MAP3K function
"endogenous ASK2 was constitutively degraded in ASK1-deficient cells, suggesting that ASK1 is required for the stability of ASK2"
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ASK2 activates ASK1 by direct phosphorylation
"ASK2 was found to activate ASK1 by direct phosphorylation"
RIP1-mediated AIP1 phosphorylation at a 14-3-3-binding site is critical for tumor necrosis factor-induced ASK1-JNK/p38 activation.
G1 to S phase transition protein 1 induces apoptosis signal-regulating kinase 1 activation by dissociating 14-3-3 from ASK1.
Connexin 43 confers resistance to hydrogen peroxide-mediated apoptosis.
Structural and functional characterization of the human protein kinase ASK1.
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ASK1 catalytic domain forms tight dimer (Kd ~0.2 uM)
"Sedimentation velocity measurements led to the determination of an apparent sedimentation coefficient corrected for water at 20°C, s20,w0, of 4.518 S, as well as the determination of a protein molecular weight of 66 kDa, which is in excellent agreement with the expected mass of an ASK1 catalytic domain dimer"
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Autophosphorylation at Thr813, Thr838, Thr842 regulates signaling
"Reporter gene assays showed that all three identified in vitro autophosphorylation sites (Thr813, Thr838, Thr842) regulate ASK1 signaling"
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Crystal structure solved in complex with staurosporine
"Here, we present the structure of the human ASK1 catalytic domain in complex with staurosporine"
Mitochondrial phosphoglycerate mutase 5 uses alternate catalytic activity as a protein serine/threonine phosphatase to activate ASK1.
A scanning peptide array approach uncovers association sites within the JNK/beta arrestin signalling complex.
CIB1 functions as a Ca(2+)-sensitive modulator of stress-induced signaling by targeting ASK1.
Dual engagement of 14-3-3 proteins controls signal relay from ASK2 to the ASK1 signalosome.
Protein phosphatase with EF-hand domains 2 (PPEF2) is a potent negative regulator of apoptosis signal regulating kinase-1 (ASK1).
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PPEF2 interacts with and negatively regulates ASK1
"We identify human PPEF2 as a novel interacting partner and a negative regulator of ASK1"
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PPEF2 suppresses H2O2-induced ASK1 activation
"PPEF2 efficiently suppressed H(2)O(2)-induced activation of ASK1"
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ASK1 activation leads to caspase-3 cleavage
"prevented ASK1-dependent caspase-3 cleavage and activation"
Stabilization of the survival motor neuron protein by ASK1.
Positive regulation of apoptosis signal-regulating kinase 1 signaling by ZPR9 protein, a zinc finger protein.
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ZPR9 is a positive regulator of ASK1
"ZPR9 was found to physically interact with apoptosis signal-regulating kinase 1 (ASK1)"
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ZPR9 stimulates ASK1-mediated JNK and p38 signaling
"Ectopic expression of wild-type ZPR9, but not an S314A/T318A mutant, stimulated ASK1 kinase activity and positively regulated ASK1-mediated signaling to both JNK and p38 kinases"
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ZPR9 destabilizes ASK1-Trx and ASK1-14-3-3 complexes
"by destabilizing complex formation between ASK1 and its negative regulators, Trx and 14-3-3"
Arginine methylation-dependent regulation of ASK1 signaling by PRMT1.
Microspherule protein 2 associates with ASK1 and acts as a negative regulator of stress-induced ASK1 activation.
Ursolic acid induces ER stress response to activate ASK1-JNK signaling and induce apoptosis in human bladder cancer T24 cells.
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ER stress induces IRE1-TRAF2-ASK1 complex formation
"Ursolic acid induces IRE1-TRAF2-ASK1 signaling complex formation to activate pro-apoptotic ASK1-JNK signaling"
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ASK1-JNK signaling promotes apoptosis in response to ER stress
"ursolic acid induces a significant ER stress response in cultured human bladder cancer T24 cells"
Crystal structures of ASK1-inhibtor complexes provide a platform for structure-based drug design.
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ASK1 has a central serine/threonine kinase domain, resolved crystallographically with ATP-competitive inhibitors bound in the ATP pocket (PDB 4BF2, 4BHN, 4BIB-4BIE)
"ASK1 is a polypeptide chain of ∼1000 residues consisting of a central serine/threonine kinase domain and N- and C-terminal coiled coil domains"
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The inhibitors occupy the ASK1 ATP binding site and interact with the kinase hinge
"the ASK1 ATP binding site is able to accommodate a range of chemical diversity and provide a platform for further structure based drug design"
Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network.
Integrative analysis of kinase networks in TRAIL-induced apoptosis provides a source of potential targets for combination therapy.
Cyclophilin A regulates JNK/p38-MAPK signaling through its physical interaction with ASK1.
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CypA binds ASK1 and negatively regulates its function
"CypA regulates apoptosis signaling-regulating kinase 1 (ASK1) through its direct binding"
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CypA negatively regulates ASK1 Ser966 phosphorylation
"CypA negatively regulates phosphorylation of ASK1 at Ser966"
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ASK1 induces caspase-3 activation and apoptosis
"ASK1 is known to induce caspase-3 activation and apoptosis"
Architecture of the human interactome defines protein communities and disease networks.
Extensive rewiring of the EGFR network in colorectal cancer cells expressing transforming levels of KRAS(G13D).
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Activation of apoptosis signal-regulating kinase 1 (ASK1) by the adapter protein Daxx.
ROS oxidize thioredoxin and activate MAP3K5
MAP3K5 phosphorylates MKK3 and MKK6
MAP3K5 (ASK1) phosphorylates Map2k4(Sek1)
Inhibition of Daxx-mediated apoptosis by heat shock protein 27.
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HSP27 prevents interaction of Daxx with Ask1, blocking Daxx-mediated apoptosis
"phosphorylated dimers of HSP27 interact with Daxx, a mediator of Fas-induced apoptosis, preventing the interaction of Daxx with both Ask1 and Fas and blocking Daxx-mediated apoptosis"
Deep research on MAP3K5 function