GO_REF:0000024

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:34800366

Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.

BDH1 is a member of the high-confidence human mitochondrial proteome (MitoCoP, 1,134 protein-coding genes), confirming its mitochondrial localization.

PMID:8679568

Wild type and mutant human heart (R)-3-hydroxybutyrate dehydrogenase expressed in insect cells.

Mature human heart BDH expressed in Sf9 insect cells is catalytically active with Km values for NAD+ and (R)-3-hydroxybutyrate similar to native mammalian enzyme, and requires phosphatidylcholine as an allosteric activator.

Reactome:R-HSA-73912

acetoacetic acid + NADH + H+ <=> beta-hydroxybutyrate + NAD+

BDH1 tetramer catalyses the ketogenesis-direction reaction, reducing acetoacetate to beta-hydroxybutyrate with NADH.

Reactome:R-HSA-73920

D-beta hydroxybutyrate+NAD+ <=> acetoacetate+NADH+H+

BDH1 tetramer catalyses the ketolysis-direction reaction, oxidising beta-hydroxybutyrate to acetoacetate with NAD+.

Reactome:R-HSA-9838035

CLPXP binds mitochondrial matrix proteins

BDH1 is engaged as one of many mitochondrial matrix substrates of the CLPXP quality-control protease; supports matrix localization but not a BDH1-specific function.

Reactome:R-HSA-9838289

CLPXP degrades mitochondrial matrix proteins

BDH1 is a substrate degraded by the CLPXP protease in the mitochondrial matrix; supports matrix localization as part of generic proteostasis.