Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Identification of the cell cycle regulator VCP (p97/CDC48) as a substrate of the band 4.1-related protein-tyrosine phosphatase PTPH1.
VCP, a weak ATPase involved in multiple cellular events, interacts physically with BRCA1 in the nucleus of living cells.
Valosin-containing protein is a multi-ubiquitin chain-targeting factor required in ubiquitin-proteasome degradation.
Comprehensive proteomic analysis of interphase and mitotic 14-3-3-binding proteins.
A membrane protein complex mediates retro-translocation from the ER lumen into the cytosol.
A novel UBA and UBX domain protein that binds polyubiquitin and VCP and is a substrate for SAPKs.
Human Fas-associated factor 1, interacting with ubiquitinated proteins and valosin-containing protein, is involved in the ubiquitin-proteasome pathway.
Proteomics of human umbilical vein endothelial cells applied to etoposide-induced apoptosis.
Valosin-containing protein phosphorylation at Ser784 in response to DNA damage.
Recruitment of the p97 ATPase and ubiquitin ligases to the site of retrotranslocation at the endoplasmic reticulum membrane.
Identification of VCP/p97, carboxyl terminus of Hsp70-interacting protein (CHIP), and amphiphysin II interaction partners using membrane-based human proteome arrays.
14-3-3 cooperates with LKB1 to regulate the activity and localization of QSK and SIK.
The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site.
Derlin-2 and Derlin-3 are regulated by the mammalian unfolded protein response and are required for ER-associated degradation.
An arginine/lysine-rich motif is crucial for VCP/p97-mediated modulation of ataxin-3 fibrillogenesis.
Conformational changes in the AAA ATPase p97-p47 adaptor complex.
The RBCC gene RFP2 (Leu5) encodes a novel transmembrane E3 ubiquitin ligase involved in ERAD.
ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.
Ufd1 is a cofactor of gp78 and plays a key role in cholesterol metabolism by regulating the stability of HMG-CoA reductase.
Identification of SVIP as an endogenous inhibitor of endoplasmic reticulum-associated degradation.
Identification of multi-SH3 domain-containing protein interactome in pancreatic cancer: a yeast two-hybrid approach.
Ubxd1 is a novel co-factor of the human p97 ATPase.
SEL1L nucleates a protein complex required for dislocation of misfolded glycoproteins.
UBXD7 binds multiple ubiquitin ligases and implicates p97 in HIF1alpha turnover.
Large-scale proteomics and phosphoproteomics of urinary exosomes.
UBXD1 is a VCP-interacting protein that is involved in ER-associated degradation.
The proapoptotic function of SAP provides a clue to the clinical picture of X-linked lymphoproliferative disease.
The otubain YOD1 is a deubiquitinating enzyme that associates with p97 to facilitate protein dislocation from the ER.
Ubiquilin and p97/VCP bind erasin, forming a complex involved in ERAD.
Structure and function of the PLAA/Ufd3-p97/Cdc48 complex.
VCP/p97 is essential for maturation of ubiquitin-containing autophagosomes and this function is impaired by mutations that cause IBMPFD.
Imbalances in p97 co-factor interactions in human proteinopathy.
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
A novel ATP-dependent conformation in p97 N-D1 fragment revealed by crystal structures of disease-related mutants.
The AAA-ATPase p97 is essential for outer mitochondrial membrane protein turnover.
The UBX protein SAKS1 negatively regulates endoplasmic reticulum-associated degradation and p97-dependent degradation.
Membrane-associated ubiquitin ligase complex containing gp78 mediates sterol-accelerated degradation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase.
Proteomic characterization of the human sperm nucleus.
A ubiquitin ligase-associated chaperone holdase maintains polypeptides in soluble states for proteasome degradation.
Hierarchical binding of cofactors to the AAA ATPase p97.
Endolysosomal sorting of ubiquitylated caveolin-1 is regulated by VCP and UBXD1 and impaired by VCP disease mutations.
A directed protein interaction network for investigating intracellular signal transduction.
The tissue-specific Rep8/UBXD6 tethers p97 to the endoplasmic reticulum membrane for degradation of misfolded proteins.
Toward an understanding of the protein interaction network of the human liver.
Defining human ERAD networks through an integrative mapping strategy.
The AAA-ATPase VCP/p97 promotes 53BP1 recruitment by removing L3MBTL1 from DNA double-strand breaks.
Valosin-containing protein (VCP/p97) is required for poliovirus replication and is involved in cellular protein secretion pathway in poliovirus infection.
NEDD8 links cullin-RING ubiquitin ligase function to the p97 pathway.
Ubiquitin-specific protease 25 functions in Endoplasmic Reticulum-associated degradation.
STT3B-dependent posttranslational N-glycosylation as a surveillance system for secretory protein.
The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Ubiquitin-dependent intramembrane rhomboid protease promotes ERAD of membrane proteins.
Proliferating cell nuclear antigen (PCNA)-binding protein C1orf124 is a regulator of translesion synthesis.
Lysine methylation of VCP by a member of a novel human protein methyltransferase family.
Valosin-containing protein (VCP/p97) is an activator of wild-type ataxin-3.
DVC1 (C1orf124) is a DNA damage-targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks.
DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage.
Spatial regulation of UBXD8 and p97/VCP controls ATGL-mediated lipid droplet turnover.
A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity.
Pathogenic VCP mutations induce mitochondrial uncoupling and reduced ATP levels.
In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
Ter94 ATPase complex targets k11-linked ubiquitinated ci to proteasomes for partial degradation.
High-speed atomic force microscopic observation of ATP-dependent rotation of the AAA+ chaperone p97.
Caveolin-1 interacts with Derlin-1 and promotes ubiquitination and degradation of cyclooxygenase-2 via collaboration with p97 complex.
Interaction between salt-inducible kinase 2 (SIK2) and p97/valosin-containing protein (VCP) regulates endoplasmic reticulum (ER)-associated protein degradation in mammalian cells.
Phosphorylation regulates VCIP135 function in Golgi membrane fusion during the cell cycle.
USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation.
Binding of OTULIN to the PUB domain of HOIP controls NF-kappaB signaling.
Ubiquitin-specific protease 19 regulates the stability of the E3 ubiquitin ligase MARCH6.
A novel mutation in VCP causes Charcot-Marie-Tooth Type 2 disease.
A proteome-scale map of the human interactome network.
Alterations in the interactome of serine/threonine protein phosphatase type-1 in atrial fibrillation patients.
Identification of ERAD components essential for dislocation of the null Hong Kong variant of alpha-1-antitrypsin (NHK).
Phospho-tyrosine dependent protein-protein interaction network.
Quantitative interaction proteomics of neurodegenerative disease proteins.
Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly.
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p97 has two discrete functions in nuclear envelope assembly: the p97-Ufd1-Npl4 complex is required for formation of a closed NE, while a separate p97-p47 complex mediates subsequent NE growth. Established by in vitro reconstitution.
"Here we show that p97, an AAA-ATPase previously implicated in fusion of Golgi and transitional endoplasmic reticulum (ER) membranes together with the adaptor p47, has two discrete functions in NE assembly. Formation of a closed NE requires the p97-Ufd1-Npl4 complex, not previously implicated in membrane fusion. Subsequent NE growth involves a p97-p47 complex."
Cdc48/p97 promotes reformation of the nucleus by extracting the kinase Aurora B from chromatin.
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Direct experimental evidence that p97 (VCP) is required for nuclear reformation after mitosis. p97 binds ubiquitylated Aurora B and extracts it from chromatin, releasing Aurora-B inhibition of chromatin decondensation and NE formation.
"Here we show that p97 stimulates nucleus reformation by inactivating the chromatin-associated kinase Aurora B. During mitosis, Aurora B inhibits nucleus reformation by preventing chromosome decondensation and formation of the nuclear envelope membrane. During exit from mitosis, p97 binds to Aurora B after its ubiquitylation and extracts it from chromatin. This leads to inactivation of Aurora B on chromatin, thus allowing chromatin decondensation and nuclear envelope formation."
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Defines ubiquitin-dependent protein extraction by Cdc48/p97 as the mechanistic basis for p97's role in nucleus formation, paralleling its well-known activity in ERAD and other quality-control pathways.
"These data reveal an essential pathway that regulates reformation of the nucleus after mitosis and defines ubiquitin-dependent protein extraction as a common mechanism of Cdc48/p97 activity also during nucleus formation."
ESCRT-III controls nuclear envelope reformation.
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The p97 AAA-ATPase, via its UFD1 and NPL4 adaptors, controls annular fusion during post-mitotic nuclear envelope reformation, while its p47 adaptor separately controls membrane delivery and NE expansion.
"The p97 AAA-ATPase controls both phases of NE reformation; in concert with its adaptor protein p47, it regulates membrane delivery and NE expansion whilst through its adaptors Nuclear Protein Like 4 (NPL4) and UFD1 it regulates annular fusion."
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Through NPL4 and UFD1, the p97 complex extracts ubiquitinated Aurora-B from chromatin to enable chromatin decondensation and membranation during NE reformation.
"through its adaptors Nuclear Protein Like 4 (NPL4) and UFD1 it regulates annular fusion. Through NPL4 and UFD1, the p97 complex extracts ubiquitinated Aurora-B, a Chromosomal Passenger Complex (CPC) component, from chromatin to allow chromatin decondensation and membranation"
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UFD1 (a p97 cofactor) is required for ESCRT-III (CHMP2A) recruitment to the forming nuclear envelope; UFD1 depletion impairs CHMP2A recruitment to the telophase NE and reduces post-mitotic nucleo-cytoplasmic compartmentalization in human cells (HeLa).
"recruitment of CHMP2A to the forming NE was impaired"
UBXN2A regulates nicotinic receptor degradation by modulating the E3 ligase activity of CHIP.
Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.
A non-canonical role of the p97 complex in RIG-I antiviral signaling.
A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Pre-emptive Quality Control Protects the ER from Protein Overload via the Proximity of ERAD Components and SRP.
Structural Basis of ATP Hydrolysis and Intersubunit Signaling in the AAA+ ATPase p97.
Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions.
2.3 Å resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition.
Chromatin-associated degradation is defined by UBXN-3/FAF1 to safeguard DNA replication fork progression.
Nucleotide-dependent conformational changes of the AAA+ ATPase p97 revisited.
Structural insights into the interaction of human p97 N-terminal domain and SHP motif in Derlin-1 rhomboid pseudoprotease.
VCP/p97 cooperates with YOD1, UBXD1 and PLAA to drive clearance of ruptured lysosomes by autophagy.
Characterization and Genetic Analyses of New Genes Coding for NOD2 Interacting Proteins.
Architecture of the human interactome defines protein communities and disease networks.
Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5.
The AAA+ ATPase p97, a cellular multitool.
Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle and Protein Quality Control.
ZFAND1 Recruits p97 and the 26S Proteasome to Promote the Clearance of Arsenite-Induced Stress Granules.
An interactome perturbation framework prioritizes damaging missense mutations for developmental disorders.
LuTHy: a double-readout bioluminescence-based two-hybrid technology for quantitative mapping of protein-protein interactions in mammalian cells.
UBXD1 is a mitochondrial recruitment factor for p97/VCP and promotes mitophagy.
Physiological and pathophysiological characteristics of ataxin-3 isoforms.
LMBR1L regulates lymphopoiesis through Wnt/beta-catenin signaling.
BIK ubiquitination by the E3 ligase Cul5-ASB11 determines cell fate during cellular stress.
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
Structure of the PUB Domain from Ubiquitin Regulatory X Domain Protein 1 (UBXD1) and Its Interaction with the p97 AAA+ ATPase.
TEX264 coordinates p97- and SPRTN-mediated resolution of topoisomerase 1-DNA adducts.
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Protein Phosphatase-1 Complex Disassembly by p97 is Initiated through Multivalent Recognition of Catalytic and Regulatory Subunits by the p97 SEP-domain Adapters.
The p97-UBXN1 complex regulates aggresome formation.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 from chromatin.
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Valosin-containing protein-regulated endoplasmic reticulum stress causes NOD2-dependent inflammatory responses.
Ribosome-associated quality-control mechanisms from bacteria to humans.
Structural remodeling of AAA+ ATPase p97 by adaptor protein ASPL facilitates posttranslational methylation by METTL21D.
Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules.
N-terminal proteoforms may engage in different protein complexes.
Analysis of proteome-wide degradation dynamics in ALS SOD1 iPSC-derived patient neurons reveals disrupted VCP homeostasis.
TRIM55 promotes noncanonical NF-kappaB signaling and B cell-mediated immune responses by coordinating p100 ubiquitination and processing.
The cryo-EM structure of the human ERAD retrotranslocation complex.
The AAA-ATPase Ter94 regulates wing size in Drosophila by suppressing the Hippo pathway.
Mapping adipocyte interactome networks by HaloTag-enrichment-mass spectrometry.
Study of Clinical Characteristics of Intellectual Disability in Morocco.
Multimodal cell maps as a foundation for structural and functional genomics.
Involvement of valosin-containing protein, an ATPase Co-purified with IkappaBalpha and 26 S proteasome, in ubiquitin-proteasome-mediated degradation of IkappaBalpha.
Dissociation of cytosolic HSF1:HSP90:HDAC6:PTGES3 upon sensing protein aggregates
SYVN1 ubiquitinates Hh C-terminal fragments
C-terminal Hh fragments are recruited to SEL1:SYVN1 at the ER membrane
VCP-catalyzed ATP hydrolysis promotes the translocation of Hh-C into the cytosol
Hh processing variants are recruited to SEL1:SYVN at the ER membrane
Hh processing variants are translocated to the cytosol in a VCP-dependent manner
Hh processing variants are ubiquitinated
SPRTN recruits VCP to monoUb:K164-PCNA associated with POLH
SPRTN:VCP-mediated release of POLH from monoUb:K164-PCNA
Exocytosis of secretory granule lumen proteins
Exocytosis of azurophil granule lumen proteins
Exocytosis of ficolin-rich granule lumen proteins
Deglycosylation complex hydrolyses N-glycans from unfolded glycoproteins
VCP-catalyzed ATP hydrolysis promotes the translocation of misfolded CFTR into the cytosol
RNF5 and RNF185 ubiquitinate misfolded CFTR
CFTR binds components of the ERAD machinery for ubiquitination and degradation
VCP-catalyzed ATP hydrolysis promotes the translocation of CFTR F508del into the cytosol
RNF5 and RNF185 ubiquitinate CFTR F508del
CFTR F508del binds components of the ERAD machinery for ubiquitination and degradation
VCPKMT (METTL21D) transfers 3xCH3 from 3xAdoMet to VCP
TMEM129 polyubiquitinates HLA (MHC class I heavy chain) bound to cytomegalovirus US11
US11:HLA binds DERL1:TMEM129:Ub:UBE2J2,UBE2K:VIMP:VCP
UBXN7:UBF1:NPLOC4:VCP hexamer binds NFE2L2:CRL3 complex
UBXN7 binds VCP hexamer:UBF1:NPLOC4
Ubiquitinated NFE2L2 is extracted from CRL3 complex for degradation
Active transport of ubiquitinated CD274 from ER to cytosol
Ubiquitination of CD274 by ERAD complex
p-S195-CD274 binds ERAD complex
ANKZF1 cleaves the peptidyl-tRNA in VCP hexamer:LTN1:NEMF:60S ribosome subunit:peptidyl-tRNA with K48polyUb-nascent peptide