GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000003

Gene Ontology annotation based on Enzyme Commission mapping

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000041

Gene Ontology annotation based on UniPathway vocabulary mapping

GO_REF:0000043

Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:human/PEX2/PEX2-deep-research-falcon.md

Falcon deep research report for human PEX2

Deep research synthesis of 2024 reviews confirms PEX2 as a subunit of the heterotrimeric PEX2/PEX10/PEX12 membrane-embedded RING E3 ubiquitin ligase complex that monoubiquitinates PEX5 at Cys11 for receptor recycling, and polyubiquitinates receptors for RADAR-pathway degradation.

"PEX2 is one of three **RING-finger peroxins** (PEX2/PEX10/PEX12) that form a **membrane-embedded E3 ubiquitin ligase** complex required for ubiquitination of the import receptors"

PMID:10528859

Defective PEX gene products correlate with the protein import, biochemical abnormalities, and phenotypic heterogeneity in peroxisome biogenesis disorders.

PEX2 E55K mutation causes mosaic peroxisomal protein import and residual beta-oxidation activity

PMID:10837480

Molecular anatomy of the peroxin Pex12p: ring finger domain is essential for Pex12p function and interacts with the peroxisome-targeting signal type 1-receptor Pex5p and a ring peroxin, Pex10p.

PEX12 RING finger interacts with PEX2 and PEX5; PEX10 and PEX12 form oligomeric complex in peroxisome membranes

PMID:11590176

Two different targeting signals direct human peroxisomal membrane protein 22 to peroxisomes.

PMP22 peroxisomal membrane targeting signals interact with PEX19

PMID:12751901

The peroxisomal membrane targeting elements of human peroxin 2 (PEX2).

PEX2 is targeted to the peroxisomal membrane via an internal targeting signal comprising TM1

PMID:1546315

A human gene responsible for Zellweger syndrome that affects peroxisome assembly.

PEX2 (PAF-1) cDNA complements Zellweger syndrome fibroblasts, restoring peroxisome assembly

PMID:18987311

The parafibromin tumor suppressor protein inhibits cell proliferation by repression of the c-myc proto-oncogene.

This paper is about PAF1 (RNA polymerase II-associated factor 1), NOT PEX2. Gene name confusion with PEX2 alias PAF1.

PMID:19946888

Defining the membrane proteome of NK cells.

Large-scale proteomics study identifying membrane proteins in NK cells

PMID:20531392

The peroxisomal receptor Pex19p forms a helical mPTS recognition domain.

PEX19 functions as receptor/chaperone for peroxisomal membrane proteins including PEX2

PMID:21525035

PEX14 is required for microtubule-based peroxisome motility in human cells.

Peroxisomal membrane proteomics identified PEX2 among peroxisomal membrane proteins

PMID:24662292

Distinct modes of ubiquitination of peroxisome-targeting signal type 1 (PTS1) receptor Pex5p regulate PTS1 protein import.

PEX10/PEX12 catalyze PEX5 monoubiquitination; PEX2 identified in the retrotranslocation channel complex

PMID:26344566

ATM functions at the peroxisome to induce pexophagy in response to ROS.

ATM phosphorylates PEX5 at Ser141 to promote ubiquitination and pexophagy; PEX2 not directly tested

PMID:27597759

PEX2 is the E3 ubiquitin ligase required for pexophagy during starvation.

PEX2 specifically (not PEX10 or PEX12) ubiquitinates PEX5 and PMP70 during amino acid starvation
PEX2 overexpression induces pexophagy via NBR1-dependent autophagy
PEX2 protein levels regulated by mTORC1 pathway
Validated in vivo in protein-restricted mice

PMID:33961781

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.

Large-scale interactome study

PMID:34903883

Peroxisomal β-oxidation acts as a sensor for intracellular fatty acids and regulates lipolysis.

PEX2 polyubiquitinates ATGL at K92 via K48-linkage for proteasomal degradation
PEX2 acts as ROS sensor with disulfide bond-mediated protein stabilization
Peroxisomal beta-oxidation derived ROS regulate PEX2 protein levels

PMID:35768507

A peroxisomal ubiquitin ligase complex forms a retrotranslocation channel.

Cryo-EM structure of PEX2/PEX10/PEX12 complex reveals retrotranslocation channel
Each subunit contributes 5 TM segments forming open channel pore
RING finger domains form cytosolic tower above channel for receptor ubiquitination

PMID:9765053

Restoration of PEX2 peroxisome assembly defects by overexpression of PMP70.

PEX2 mutations cause defective peroxisome assembly in CHO cells
Human PEX2 expression restores peroxisome biogenesis and VLCFA beta-oxidation

Reactome:R-HSA-8953917

PEX2:PEX10:PEX12 binds PEX5S,L (in PEX5S:PEX13:PEX14) and Ub:UBE2D1,2,3

Reactome:R-HSA-8953946

PEX2:PEX10:PEX12 monoubiquitinates PEX5S,L at cysteine-11

Reactome:R-HSA-9033235

Cargo of PEX5S,L translocates from the cytosol to the peroxisomal matrix

Reactome:R-HSA-9033236

PEX5S,L:Cargo binds PEX13:PEX14:PEX2:PEX10:PEX12 (Docking and Translocation Module)

Reactome:R-HSA-9033485

PEX2:PEX10:PEX12 monoubiquitinates PEX5L at cysteine-11

Reactome:R-HSA-9033499

PEX1:PEX6:PEX26:ZFAND6 dissociates Ub:PEX5L and PEX7 from PEX14:PEX13:PEX2:PEX10:PEX12 and translocates PEX5L and PEX7 from the peroxisomal membrane to the cytosol

Reactome:R-HSA-9033514

Cargo of PEX5L:PEX7 translocates from the cytosol to the peroxisomal matrix

Reactome:R-HSA-9033516

PEX2:PEX10:PEX12:Ub:PEX5L:PEX7:PEX13:PEX14 binds PEX1:PEX6:PEX26 and ZFAND6

Reactome:R-HSA-9033527

PEX2:PEX10:PEX12 binds PEX5L (in PEX5L:PEX7:PEX13:PEX14:PEX2:PEX10:PEX12) and Ub:UBE2D1,2,3

Reactome:R-HSA-9033533

PEX2:PEX10:PEX12:Ub:PEX5S,L:PEX13:PEX14 binds PEX1:PEX6:PEX26 and ZFAND6

Reactome:R-HSA-9603775

PEX3:PEX19:class I PMP dissociates

Reactome:R-HSA-9603784

PEX19:class I PMP binds PEX3

Reactome:R-HSA-9603804

PEX19 binds class I peroxisomal membrane proteins