Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Global analysis of protein localization in budding yeast.
Rational extension of the ribosome biogenesis pathway using network-guided genetics.
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TSR4 was identified as a ribosome biogenesis factor
"We experimentally evaluated >100 candidate yeast genes in a battery of assays, confirming involvement of at least 15 new genes, including previously uncharacterized genes (YDL063C, YIL091C, YOR287C, YOR006C/TSR3, YOL022C/TSR4)."
Tsr4 and Nap1, two novel members of the ribosomal protein chaperOME.
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Tsr4 is a direct, cotranslational Rps2/uS5 chaperone that promotes client solubility.
"We report the identification of Nap1 and Tsr4 as direct binding partners of Rps6 and Rps2, respectively. Both factors promote the solubility of their r-protein clients in vitro."
Tsr4 Is a Cytoplasmic Chaperone for the Ribosomal Protein Rps2 in Saccharomyces cerevisiae.
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Tsr4 is a cytoplasmic chaperone dedicated to Rps2
"Here, we report that Tsr4 cotranslationally associates with Rps2. Rps2 harbors a eukaryote-specific N-terminal extension that is critical for its interaction with Tsr4."
The N-terminal extension of ribosomal protein Rps2 shapes its early fate through Chaperone-Importin competition and arginine methylation.
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Tsr4 competes with Pse1 for the Rps2 N-terminal extension, while Rps2 methylation can occur on the Tsr4-associated pre-import client.
"We demonstrate that Rps2 is already methylated while associated with Tsr4, indicating that methylation occurs at an early stage of the Rps2 assembly path prior to nuclear import. We further show that the chaperone Tsr4 and the importin Pse1 compete for binding to the Rps2 N-terminal extension."
Falcon deep research report for TSR4
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TSR4 is a cytoplasmic, client-specific uS5/Rps2 chaperone.
"**Molecular function (best-supported):** Tsr4 binds **nascent Rps2/uS5** co-translationally in the cytoplasm and acts as a **client-specific chaperone** to stabilize Rps2 and support 40S biogenesis (black2019tsr4isa pages 1-3, rossler2019tsr4andnap1 pages 12-13)."