Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Combined Automated Annotation using Multiple IEA Methods
Involvement of the rml locus in core oligosaccharide and O polysaccharide assembly in Pseudomonas aeruginosa.
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rmlC mutants make a truncated LPS core lacking the O-antigen attachment point; L-Rha is the core receptor for O polysaccharide.
"These results provide the genetic and structural evidence that L-Rha is the receptor on the P. aeruginosa LPS core for the attachment of O polysaccharides."
RmlC, a C3' and C5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation.
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Structures of RmlC (including P. aeruginosa RmlC) with product and product mimics, and assays of site-directed mutants, assign the catalytic residues of the C3'/C5' epimerase.
"Here we report the structure of RmlC from several organisms in complex with product and product mimics."
UniProt record Q9HU21 (rmlC)