Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
A cell-counting factor regulating structure size in Dictyostelium.
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Countin is a ~40 kDa secreted, hydrophilic protein with a signal sequence, purified as part of a ~450 kDa counting-factor complex.
"The predicted protein, which we have named countin, is hydrophilic, has a potential signal sequence upstream of where we observe the amino terminus of the secreted protein"
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Disrupting the countin gene abolishes counting-factor secretion so aggregation streams do not break up, giving huge fruiting bodies.
"there is no detectable secretion of counting factor, and the aggregation streams do not break up"
A precise group size in Dictyostelium is generated by a cell-counting factor modulating cell-cell adhesion.
A cell number-counting factor regulates group size in Dictyostelium by differentially modulating cAMP-induced cAMP and cGMP pulse sizes.
Cells respond to and bind countin, a component of a multisubunit cell number counting factor.
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Recombinant countin binds ~53 high-affinity cell-surface sites and triggers rapid responses including increased motility, decreased adhesion and increased F-actin polymerization.
"Recombinant countin increases cell motility, decreases cell-cell adhesion"
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Cells have a small number of high-affinity countin-binding sites.
"vegetative and developing cells have approximately 53 cell-surface sites that bind countin"
CF45-1, a secreted protein which participates in Dictyostelium group size regulation.
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Countin-null cells have elevated cytosolic glucose, higher adhesion and lower motility, forming large groups.
"cells lacking countin or CF50 have higher glucose levels, higher cell-cell adhesion, and lower motilities"
Exposure of cells to a cell number-counting factor decreases the activity of glucose-6-phosphatase to decrease intracellular glucose levels in Dictyostelium discoideum.
A protein in crude cytosol regulates glucose-6-phosphatase activity in crude microsomes to regulate group size in Dictyostelium.
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CF represses internal glucose by increasing the Km of glucose-6-phosphatase.
"The CF signal transduction pathway involves CF-repressing internal glucose levels by increasing the K(m) of glucose-6-phosphatase"
Response of Dictyostelium discoideum to UV-C and involvement of poly (ADP-ribose) polymerase.
Collective signalling drives rapid jumping between cell states.
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Developmental gene expression jumps are driven by collective cAMP oscillations; ctnA is among cAMP-responsive genes but is not shown to act within the cAMP receptor pathway.
"the jump coincides with the onset of collective oscillations of cAMP"
Trafficking of adhesion and aggregation-modulating proteins during the early stages of Dictyostelium development.
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CtnA localizes to cytoplasmic vesicles and punctae and is secreted in a glycosylation- and signal-peptide-dependent manner.
"During growth and starvation, CtnA localizes to cytoplasmic vesicles and punctae"