Original article, Methods and Figure 4.
Methods and Figure 1 use Git5 with a Git3 cytoplasmic fragment and Gpa2 in a yeast reporter assay. The donor supports assembly, with no beta-catalyzed nucleotide exchange claim.
The original uses native bovine beta1–gamma1 with recombinant alpha-T; some structures include engineered alpha and nanobody. Alpha-interface mutagenesis informs the donor mechanism without asserting a direct GNB4 catalytic assay.
Author-upload original, Table 2 row:
Q9HAV0 Guanine nucleotide binding protein beta subunit 4 0.81 ±0.79 0.44 1.25 ±0.17 1.00 1.12 ±0.37 0.89
The full author-upload article's Methods and Tables 1–2 describe adult postmortem subjects, not developmental stages. Q9HAV0 appears in Table 2; its high reported probability denotes increased abundance, not a conventional nonsignificant p-value.
Figure 3B was visually inspected: GNB4 appears with Radil and Radil-DeltaPDZ in all three purifications. Mouse bait and human cell host are distinct. No target peptide supplement replay is claimed.
Original supplementary archive, file 1-s2.0-S2211124720310354-mmc3.xlsx, sheet NN1.0, rows 3846–3848.
Supplement mmc3, NN1.0 rows 3846–3848, and IntAct array/pooling/validated two-hybrid records identify the three partners. Gene-level supplement identifiers do not establish full clone sequences or universal validation by another assay.
Publisher Table 1, beta4 row:
G protein β4 subunit | IPI00012451 | 3 | 339 | 0 | 13 | 57.8 | 67 | 1.96 | 0.023
Publisher Table 1 and purification Methods were read. The named beta4 row meets the paper's enrichment criterion. Supplemental Table S3 remained inaccessible; the historical 339-residue table entry does not replace the current 340-residue declaration.
Original supplementary workbook, sheet SI Table 2, row 629.
Authentic SI Table 2 row 629 groups P62879, Q9HAV0 and P16520. Pooled EPS-urine vesicles and possible co-isolation limit interpretation; accession-specific peptide uniqueness was not established.
Actual mouse P29387 records carry four experimental SynGO synapse annotations. The paper was available as an abstract only; that limitation is retained without alleging a wrong-gene curator error.
All eight event paths reach Q9HAV0 itself. Folding events describe a client; mixed plasma-membrane parent complexes retain cytosolic target leaves. Location is never inferred from an unrelated nucleotide or released co-chaperone.
The exact 340-residue target is AN218/PTN002500459 in PTHR19850, below AN0/PTN000457487. Incoming and later donor versions remain separate. No recorded negative assertion lies on its path; this observation alone is not proof of function.
The development term defines formation through maturity and has is_a parent GO:0048857 and part_of parent GO:0030901. GPCR signaling is an is_a descendant of signal transduction. The activity-location qualifiers are retained literally.
The accompanying evidence JSON preserves the 22 raw objects, exact exporter joins, official identity, original source pins and target-specific ancestry/participant paths. It records access limits without modifying cached publications. No new GO annotation is proposed.