Gene Ontology annotation through association of InterPro records with GO terms
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry for glgX (Q88FN4)
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The unreviewed record assigns EC 3.2.1.33, GlgX-specific GH13 signatures, GO:0004135, and glycogen catabolic process.
Role of the Escherichia coli glgX gene in glycogen metabolism.
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E. coli GlgX was experimentally shown to be an isoamylase-type debranching enzyme selective for branches of three or four glucose residues and predominantly involved in glycogen catabolism.
"debranching enzyme with high specificity for hydrolysis of chains consisting of three or four glucose residues"
Trehalose and α-glucan mediate distinct abiotic stress responses in Pseudomonas aeruginosa.
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Purified P. aeruginosa GlgX released predominantly DP4 and less DP5+ maltooligosaccharide from glycogen, directly supporting short-chain bacterial glycogen debranching in a close Pseudomonas homolog.
"MALDI-MS showed that the enzyme preferentially liberated a maltooligosaccharide with a DP of 4 and significantly less material with a DP of 5 and above (Fig 7), supporting its role as a debranching enzyme with a short chain-length specificity."