GO_REF:0000033

Annotation inferences using phylogenetic trees

SwrD is conserved across Firmicutes, Clostridia, Spirochaetes, and Deltaproteobacteria
SwrD homologs often co-occur adjacent to motAB in diverse bacteria

PMID:29061663

SwrD (YlzI) Promotes Swarming in Bacillus subtilis by Increasing Power to Flagellar Motors.

swrD (formerly ylzI) encodes a small ~71 aa protein in the fla-che operon

"Here we report a new gene, swrD, located within the 32 gene fla-che operon dedicated to flagellar biosynthesis and chemotaxis"
swrD deletion abolishes swarming but cells retain swimming ability with reduced speed

"which when mutated abolished swarming motility...Mutation of swrD reduced swimming speed"
swrD deletion reduces single-flagellum torque approximately 6-fold

"Mutation of swrD reduced swimming speed and torque of tethered flagella"
SwrD does not affect flagellar number, flagellar gene expression, or surfactant production

"SwrD was not required for surfactant production, flagellar gene expression, or an increase in flagellar number"
SwrD acts at the level of MotAB stator activity, not abundance

"Instead, SwrD was required to increase flagellar power"
MotAB overexpression rescues all swrD-related phenotypes

"all swrD-related phenotypes were restored when the stator subunits MotA and MotB were overexpressed either by spontaneous suppressor mutations or by artificial induction"
SwrD is located adjacent to fliL with overlapping ORFs

"Here we report a new gene, swrD, located within the 32 gene fla-che operon dedicated to flagellar biosynthesis and chemotaxis"

PMID:25313396

FlgM is secreted by the flagellar export apparatus in Bacillus subtilis.

This paper is about FlgM secretion and does NOT mention swrD

"Here we demonstrate that FlgM is also secreted in the Gram-positive bacterium Bacillus subtilis and is degraded extracellularly by the proteases Epr and WprA"
Erroneously cited as evidence for swrD annotation in GOA

"FlgM is secreted by the flagellar export apparatus in Bacillus subtilis"
Paper focuses on FlgM, FliF, FliG, FliO, FliP, FliQ, FliR, FlhA, FlhB, FliK

"FlgM secretion is strongly enhanced by, but does not strictly require, hook-basal body completion and instead demands a minimal subset of flagellar proteins that includes the FliF/FliG basal body proteins, the flagellar type III export apparatus components FliO, FliP, FliQ, FliR, FlhA, and FlhB, and the substrate specificity switch regulator FliK"

file:BACSU/swrD/swrD-deep-research-falcon.md

Deep research on swrD function