GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping

PMID:20676083

A ribosome-associating factor chaperones tail-anchored membrane proteins.

Identifies the Bat3/TRC35(C7ORF20)/Ubl4A complex that is recruited to ribosomes, binds TA-protein TMDs, and facilitates TA protein capture by TRC40 for targeting.

PMID:21516116

Next-generation sequencing to generate interactome datasets.

PMID:21636303

A ubiquitin ligase-associated chaperone holdase maintains polypeptides in soluble states for proteasome degradation.

The Bag6/Ubl4A/Trc35 complex acts as a holdase that chaperones retrotranslocated polypeptides to the proteasome to improve ERAD efficiency; Trc35 keeps Bag6 outside the nucleus for ERAD engagement.

PMID:24722188

Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism.

PMID:25416956

A proteome-scale map of the human interactome network.

PMID:25535373

Bag6 complex contains a minimal tail-anchor-targeting module and a mock BAG domain.

The minimal Bag6 complex facilitates TA substrate transfer from SGTA to TRC40, the post-translational ER-targeting loading step.

PMID:28514442

Architecture of the human interactome defines protein communities and disease networks.

PMID:29042515

Structural basis for regulation of the nucleo-cytoplasmic distribution of Bag6 by TRC35.

TRC35 binding occludes the BAG6 nuclear localization sequence to retain BAG6 in the cytosol and protects TRC35 from RNF126-mediated ubiquitylation/degradation.

PMID:32296183

A reference map of the human binary protein interactome.

PMID:32395830

Mutations in GET4 disrupt the transmembrane domain recognition complex pathway.

Biallelic GET4 missense variants reduce all three TRC proteins by 70-90% and impair targeting of the TA protein syntaxin 5, causing a congenital disorder of glycosylation.

PMID:33961781

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.

PMID:34887561

Structural insights into metazoan pretargeting GET complexes.

Cryo-EM of the metazoan pretargeting GET complex; Get4(TRC35) directly contacts Get3/TRC40 and the Get3 helix-8/Get4 C-terminus form a composite lid over the substrate chamber, facilitating TA transfer from SGTA to Get3.

PMID:40205054

Multimodal cell maps as a foundation for structural and functional genomics.

PMID:37747814

Proteotoxic stresses stimulate dissociation of UBL4A from the tail-anchored protein recognition complex.

Disease-associated polyQ inclusions, proteasome inhibition, and CCCP-induced mitochondrial depolarization stimulate dissociation of UBL4A from BAG6 within the BAG6/UBL4A/GET4(TRC35) complex, indicating the TA-recognition complex is remodeled under proteotoxic stress.

PMID:35409131

Targeting of Proteins for Translocation at the Endoplasmic Reticulum.

Reviews the mammalian TRC/GET pre-targeting machinery, describing TRC35(GET4) within ribosome-associated BAG6 complexes that enhance recruitment of TA-handling factors and promote handover of TA clients to TRC40.

file:human/GET4/GET4-uniprot.txt

UniProt entry Q7L5D6 (GET4_HUMAN), Golgi to ER traffic protein 4 homolog / TRC35

Component of the BAG6/BAT3 complex (BAG6 + UBL4A + GET4/TRC35); interacts directly with BAG6 (retaining it in the cytosol) and with GET3; cytosolic scaffold of the TA pre-targeting pathway.