Influence of a putative ECF sigma factor on expression of the major outer membrane protein, OprF, in Pseudomonas aeruginosa and Pseudomonas fluorescens.
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Primer extension of sigX-disrupted versus wild-type RNA identifies a SigX-dependent oprF promoter with an ECF-type -35 region.
"monocistronic transcription of oprF occurs from two overlapping promoters, one that is SigX-dependent and resembles ECF sigma factor promoters in its minus-35 region"
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The sigX mutant has a reduced logarithmic-phase growth rate in rich medium.
"which included a markedly reduced logarithmic-phase growth rate in rich medium"
Identification of the alternative sigma factor SigX regulon and its implications for Pseudomonas aeruginosa pathogenicity.
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ChIP-seq maps SigX binding regions genome-wide and defines its DNA binding motif.
"the combination of transcriptional data with chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing (ChIP-seq) led to the identification of the DNA binding motif of SigX"
Transcription of the oprF gene of Pseudomonas aeruginosa is dependent mainly on the SigX sigma factor and is sucrose induced.
Extracytoplasmic function sigma factors in Pseudomonas aeruginosa.
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Review grouping the P. aeruginosa ECF sigma factors by function.
"the iron starvation σ (PvdS, FpvI, HasI, FecI, FecI2 and others), the cell wall stress ECFσ AlgU, SigX and SbrI, and the unorthodox σVreI involved in the expression of virulence"
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
UniProt entry for sigX (PA1776)