rod (Rough deal; Q9V9W7) notes

Identity

• KNTC1/ROD family (PANTHER PTHR15688), large ~2089 aa beta-propeller/alpha-solenoid protein; largest RZZ subunit.

Literature journal

• rod mutants missegregate sister chromatids; Rod needed to recruit Zw10 and dynein PMID:10523511. Dynamic localization PMID:10523511.
• Required for SAC PMID:11146659.
• Rod/Zw10 complex PMID:11590237.
• GFP-Rod streams from kinetochores along microtubules; proposed corona component PMID:14711415.
• Mad2 recruitment PMID:15886105.
• rod(Z3) maternal-effect allele: RZZ not recruited in syncytial embryos, no SAC PMID:25616898.
• Golgi role [PMID:22685323 "Rod is concentrated at the Golgi but not at the ER"; "Rod plays a Golgi-related function that is not required for spermatocyte cytokinesis"].
• Rab18 binding PMID:25453831.
• rod RNAi used as tool to induce CIN PMID:26859353 - downstream consequences only.

Curation thoughts

• Core: RZZ subunit acting as kinetochore adaptor recruiting Spindly-dynein and Mad1-Mad2; SAC; chromosome segregation.
• Deep research (falcon) failed (OOM) on first attempt.

Deep research (falcon) additions

• Retry produced rod-deep-research-falcon.md: Rod is a kinetochore scaffold/adaptor ["no catalytic reaction or transported substrate is established for Rod"]; Rod is required for robust Zw10-Zwilch association and Mad1-Mad2 accumulation; Spindly links RZZ to dynein.