Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
DROME/Rhp/Rhp-uniprot.txt
The RhoA-binding protein, rhophilin-2, regulates actin cytoskeleton organization.
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Human Rhophilin-1 and Rhophilin-2 bind both GDP- and GTP-loaded RhoA in vitro, and Rhophilin-2 expression disassembles HeLa stress fibres, requiring its Bro1 and PDZ domains, without lowering RhoA-GTP.
"interacted with both GDP- and GTP-bound RhoA in vitro"
Rhophilin-1 is a key regulator of the podocyte cytoskeleton and is essential for glomerular filtration.
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Mouse Rhophilin-1 is a podocyte plasma-membrane-enriched protein that inhibits Rho-dependent stress fiber formation, and knockout mice develop albuminuria with foot process effacement. Source of the IDA donor annotations for the PAINT node.
"Taken together, these results indicate that Rhophilin-1 is an inhibitor of Rho-dependent stress fiber formation in vitro"
Molecular requirements for actin-based lamella formation in Drosophila S2 cells.
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In an S2-cell RNAi screen of actin regulators, rhophilin depletion was listed under no effect on lamellae, and protein depletion was not confirmed for rhophilin.
"Rab5Rac1/2RhoBTBRhoLrhophilinsanpodospireb"
STRIPAK integrates upstream signals to initiate the Hippo kinase cascade.
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In mammalian cells active RhoA dissociates rhophilin and NF2/Kibra from STRIPAK, promoting Hippo kinase activation; not tested in Drosophila.
"active RhoA binds and dissociates rhophilin and NF2/Kibra from STRIPAK"
Rhp pairwise identity to vertebrate rhophilins (re-review)