Annotation inferences using phylogenetic trees
Characterization of Arabidopsis and rice DWD proteins and their roles as substrate receptors for CUL4-RING E3 ubiquitin ligases.
Rational association of genes with traits using a genome-scale gene network for Arabidopsis thaliana.
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Two independent Arabidopsis drs1 alleles have deficient water retention and ABA-insensitive transpiration, establishing the target-specific phenotype.
"An independent T-DNA allele (Salk_149366C) that we designate as drs1-2 exhibited the same phenotypes in relative water content following drought and ABA-insensitive water transpiration"
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The target mutation affects ABA-dependent leaf water loss; this does not establish an ABA receptor or a universal effect on all ABA responses.
"The mutant was insensitive to ABA on water loss whereas the wild type lost significantly less water in the presence of 10 μM ABA"
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The primary DRS1 study identifies a DWD motif but presents DDB1-CUL4 substrate-receptor function as a family-based hypothesis rather than a demonstrated DRS1 complex.
"Drs1 is a WD-40 repeat family protein containing a DWD (DDB1 binding WD40) motif"
WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage.
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Human WDR76 has chromatin-associated localization and a rapid DNA-damage response; these observations define homolog evidence, not an Arabidopsis assay.
"WDR76 co-localizes to puncta with the heterochromatin
proteins CBX1 and CBX5"
5-hydroxymethylcytosine is not present in appreciable quantities in Arabidopsis DNA.
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Sensitive assays constrain the amount of genomic 5hmC in the Arabidopsis tissues and genotypes tested, arguing against an assumed abundant canonical 5hmC signaling system.
"Enzymatic radiolabeling and mass spectrometry, the most
sensitive methods for detection that we used, failed to detect
5-hydroxymethylcytosine in A. thaliana genomic DNA"
Frozen Wdr76 prediction donor with inferred functional evidence
Frozen exact-input ProtNLM output and UniProt sequence for Q9SAI7