Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Integrin-mediated adhesion in the unicellular holozoan <i>Capsaspora owczarzaki</i>
-
coVIN forms distinct patches along the filopodia of adherent cells.
"Distinct patches of vinculin are observed in the filopodia."
-
coVIN and integrin beta2 patches partially overlap.
"Co-immunostaining showed a partial overlap, some patches contain both, whereas others have just one of the proteins"
-
Vinculin staining in filopodia increases on fibronectin.
"The overall fluorescence intensity of integrin β2 and vinculin staining within filopodia increased"
Integrin-Mediated Adhesion in the Unicellular Holozoan Capsaspora owczarzaki.
Analysis of a vinculin homolog in a sponge (phylum Porifera) reveals that vertebrate-like cell adhesions emerged early in animal evolution.
-
Sponge vinculin is autoinhibited for F-actin binding, and talin relieves the inhibition.
"Thus, binding of talin to Op vinculin D1 releases autoinhibition for F-actin binding, similar to the behavior of vertebrate vinculin."
-
Sponge vinculin lacks domain D2.
"Op vinculin lacks domain 2, which typically distinguishes vinculin from α-catenin in other animals"
Ancient origin of the integrin-mediated adhesion and signaling machinery.
-
Vinculin and the other adhesome scaffolds are found across unikonts.
"all scaffolding proteins involved in the integrin adhesion apparatus (that is, α-actinin, vinculin, paxillin, and talin) are common among unikonts"