Characterization of an ECF sigma factor protein from Pseudomonas aeruginosa.
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Purified His-PvdS directs E. coli core RNAP to pyoverdine promoters and supports in vitro transcription; PvdS co-purifies with P. aeruginosa RNAP.
"The purified protein caused core RNA polymerase from Escherichia coli to bind specifically to the promoters of pyoverdine synthesis genes and enabled transcription from these promoters in vitro"
Functional analysis of PvdS, an iron starvation sigma factor of Pseudomonas aeruginosa.
Cloning and characterization of pvdS, a gene required for pyoverdine synthesis in Pseudomonas aeruginosa: PvdS is probably an alternative sigma factor.
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pvdS is required for, and sufficient in E. coli for, expression from pyoverdine gene promoters.
"The pvdS gene is required for expression from promoters of at least two pyoverdine synthesis genes and can cause expression from these promoters in Escherichia coli, where they are otherwise inactive"
Analysis of promoters recognized by PvdS, an extracytoplasmic-function sigma factor protein from Pseudomonas aeruginosa.
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PvdS-RNAP requires the IS box to bind and initiate transcription at pvd promoters; the IS box is the PvdS -35 element.
"Band shift assays and in vitro transcription experiments showed that a complex of PvdS and core RNA polymerase required the presence of an IS box in order to bind to and initiate transcription from pvd promoters"
A positive regulatory gene, pvdS, for expression of pyoverdin biosynthetic genes in Pseudomonas aeruginosa PAO.
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A pvdS null mutant makes no pyoverdine and does not transcribe pvd promoters.
"A genomic pvdS mutant of PAO, constructed by allelic exchange mutagenesis, produced no pyoverdin and did not allow transcription from the pvd promoters"
Iron-regulated transcription of the pvdA gene in Pseudomonas aeruginosa: effect of Fur and PvdS on promoter activity.
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pvdA promoter activity requires PvdS.
"Transcription from the pvdA promoter did not occur in a heterologous host unless in the presence of the pvdS gene in trans and was abrogated in a pvdS mutant of P. aeruginosa"
Regulation of the Pseudomonas aeruginosa toxA, regA and ptxR genes by the iron-starvation sigma factor PvdS under reduced levels of oxygen.
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PvdS-RNAP binds the toxA, regA and ptxR promoters; pyoverdine production requires PvdS.
"optimal toxA, regA and pvdD expression and pyoverdine production required PvdS"
Intracellular levels and activity of PvdS, the major iron starvation sigma factor of Pseudomonas aeruginosa.
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About 30% of PvdS is membrane-retained; pyoverdine signalling releases it to the cytosol.
"Pyoverdine signalling does not affect the PvdS cellular content but facilitates PvdS release from the membrane"
Global regulation of quorum sensing and virulence by VqsR in Pseudomonas aeruginosa.
Gene repression by the ferric uptake regulator in Pseudomonas aeruginosa: cycle selection of iron-regulated genes.
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pvdS is a direct Fur target, an iron-repressed gene.
"While four PIGs are identical to already known genes (pfeR, pvdS, tonB, and fumC, respectively)"
Extracytoplasmic function sigma factors in Pseudomonas aeruginosa.
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Review grouping the P. aeruginosa ECF sigma factors by function.
"the iron starvation σ (PvdS, FpvI, HasI, FecI, FecI2 and others), the cell wall stress ECFσ AlgU, SigX and SbrI, and the unorthodox σVreI involved in the expression of virulence"
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
UniProt entry for pvdS (PA2426)