Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple Source Sequence Data
CulB, a putative ubiquitin ligase subunit, regulates prestalk cell differentiation and morphogenesis in Dictyostelium spp.
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Activating PKA by disrupting the pkaR regulatory subunit gene enhances prestalk/stalk cell differentiation, implicating PKA in stalk cell fate control.
"Activation of cyclic AMP-dependent protein kinase (PKA) by disruption of the regulatory subunit gene (pkaR) or by overexpression of the catalytic subunit gene (pkaC) enhances the prestalk/stalk cell differentiation phenotype of the culB mutant"
Mutation of protein kinase A causes heterochronic development of Dictyostelium.
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The rde/rdeC lesion maps to the PKA regulatory subunit; inactivating it unleashes constitutive catalytic-subunit activity and premature terminal differentiation.
"Inactivation of this protein results in the unrestrained activity of the catalytic subunit, so prematurely triggering terminal cell differentiation"
Surrogate hosts: protozoa and invertebrates as models for studying pathogen-host interactions.
Chemoattractant signaling in dictyostelium discoideum.
Molecular and functional analysis of the dictyostelium centrosome.
Culmination in Dictyostelium is regulated by the cAMP-dependent protein kinase.
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PKA activity is required for the movement of prestalk cells at culmination and their differentiation into stalk cells.
"the activity of PKA is necessary for the altered pattern of movement of prestalk cells at culmination and their differentiation into stalk cells"
A cAMP-dependent protein kinase is present in differentiating Dictyostelium discoideum cells.
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The purified regulatory subunit inhibits catalytic-subunit activity in a cAMP-reversible manner and binds cAMP with high specificity (Kd 4 nM).
"This subunit inhibits the activity of purified catalytic subunit from beef heart protein kinase; the inhibition is reversed upon addition of cAMP"
Multiple roles for cAMP-dependent protein kinase during Dictyostelium development.
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The Dictyostelium PKA holoenzyme is a single R plus single C subunit, and R-subunit mutants dissect the cAMP-binding and C-inhibition functions.
"we have constructed mutants of the R subunit that are defective in cAMP binding, in inhibition of the C subunit, or in both functions"
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PKA-deficient cells are defective in cAMP relay and fail to express postaggregative genes.
"a number of postaggregative genes are not expressed in PKA- cells"
Expression and properties of the regulatory subunit of Dictyostelium cAMP-dependent protein kinase encoded by lambda gt11 cDNA clones.
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Recombinant R subunit retains high-affinity cAMP binding and the ability to inhibit the catalytic subunit through protein-protein interaction.
"the ability to inhibit the catalytic (C) subunit through protein-protein interaction"
An unusual adenosine cyclic 3',5'-phosphate-dependent protein kinase from Dictyostelium discoideum.
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The Dictyostelium PKA holoenzyme is an unusual dimer of one regulatory and one catalytic subunit, and the R subunit carries a single high-affinity cAMP-binding site.
"holoenzyme is a dimer consisting of one regulatory and one catalytic subunit"
Adenylate cyclase A acting on PKA mediates induction of stalk formation by cyclic diguanylate at the Dictyostelium organizer.
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PKA acts downstream of c-di-GMP to induce stalk gene expression; PKA activation bypasses the c-di-GMP requirement.
"knockdown of cAMP-dependent protein kinase (PKA) activity in prestalk cells reduced stalk gene induction by c-di-GMP, whereas PKA activation bypassed the c-di-GMP requirement for stalk gene expression"
Curcumin affects gene expression and reactive oxygen species via a PKA dependent mechanism in Dictyostelium discoideum.
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Curcumin causes a PKA-dependent decrease in catalase A and superoxide dismutases, increasing reactive oxygen species.
"a protein kinase A dependent decrease in catalase A and three superoxide dismutase enzymes"
Protein kinase A is a positive regulator of spore coat gene transcription in Dictyostelium.
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A dominant PKA inhibitor in prespore cells reduces transcription of the spore coat genes cotB and cotC, showing PKA positively regulates spore coat gene expression.
"expression of a dominant inhibitor of the cAMP dependent protein kinase (PKA) in prespore cells greatly reduces the transcription rates of the cotB and cotC genes"
An unusual catalytic subunit for the cAMP-dependent protein kinase of Dictyostelium discoideum.
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The pkaC catalytic subunit is physically associated with the regulatory subunit, and this association inhibits catalytic activity in a cAMP-reversible manner.
"It is physically associated with the regulatory subunit, and this association results in an inhibition of the catalytic activity which is reverted by cAMP"
Induction of terminal differentiation of Dictyostelium by cAMP-dependent protein kinase and opposing effects of intracellulr and extracellular cAMP on stalk cell differentiation.
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Ectopic expression of the PKA catalytic subunit under cell-type promoters causes ectopic terminal differentiation, demonstrating PKA drives terminal differentiation and gene expression.
"Expression of the catalytic (C) subunit of the cAMP-dependent protein kinase (PKA) of Dictyostelium under the control of heterologous, cell-type-specific promoters causes ectopic terminal differentiation"
A cAMP-phosphodiesterase controls PKA-dependent differentiation.
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The RegA cAMP-phosphodiesterase is stimulated by binding the PKA regulatory subunit (PKA-R), and PKA activity acts at a checkpoint for terminal differentiation.
"A cAMP-specific phosphodiesterase was found that is stimulated by binding to the regulatory subunit of cAMP-dependent protein kinase, PKA-R"