GPD1 (YDL022W, UniProt Q00055) notes

Activity / role

• NADH-dependent cytosolic GPDH, key enzyme of glycerol synthesis PMID:8196651
• Osmotic role PMID:8196651; HOG-regulated PMID:8196651
• GPDH (not Gpp) is rate-limiting for glycerol flux PMID:11676566
• Reaction EC 1.1.1.8, RHEA:11092 [UniProt:Q00055]

Localization

• Cytosol + peroxisome PMID:15210723
• PTS2/Pex7 import PMID:20026609; nucleus under osmotic stress PMID:20026609
• Piggyback carrier for Pnc1p PMID:26276932
• Mitochondrion HDA (PMID:24769239) unsupported by targeted work -> marked over-annotated.

Curation observations

• [NAD(P)+] GPDH rows (IBA/IMP/RCA) -> MODIFY to NAD+-specific GO:0141152.
• InterPro2GO 'glycerol-3-phosphate catabolic process' is direction-wrong for this enzyme in vivo -> MODIFY to G3P biosynthetic process.
• YeastPathways superpathway RCA 'phospholipid biosynthetic process' marked over-annotated; PA biosynthesis kept non-core.