PMID:12910271

Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica

Establishes the complete genome sequence of Bordetella pertussis strain Tohama I, including locus BP0922.

PMID:17567742

The three-dimensional crystal structure of the PrpF protein of Shewanella oneidensis complexed with trans-aconitate: insights into its biological function.

S. oneidensis PrpF has a DapF-like fold, isomerizes trans-aconitate to cis-aconitate in vitro, and uses Lys73 (with Cys107 nearby) at the active site. It is required only in bacteria that use AcnD. PrpF and Mii share only 39% identity.

PMID:29145506

The PrpF protein of Shewanella oneidensis MR-1 catalyzes the isomerization of 2-methyl-cis-aconitate during the catabolism of propionate via the AcnD-dependent 2-methylcitric acid cycle.

SoPrpF isomerizes the AcnD product (putatively 4-methyl-cis-aconitate) to 2-methyl-cis-aconitate, the aconitase substrate in the 2-methylcitrate cycle.

file:BORPE/Q7VZI5/Q7VZI5-uniprot.txt

UniProt entry Q7VZI5

Records the PrpF-like domain (IPR007400), the PrpF Pfam domain (PF04303), PANTHER PTHR43709:SF2 and the diaminopimelate epimerase-like fold. The recommended name is generated by ProtNLM, and the family similarity note rests on ARBA. Neither is used as evidence here.

file:BORPE/Q7VZI5/Q7VZI5-deep-research-falcon.md

Deep research summary for Q7VZI5

States that no organism-specific experimental literature exists for BP0922.

file:BORPE/Q7VZI5/Q7VZI5-bioinformatics/RESULTS.md

Q7VZI5 PTHR43709 comparison, active-site mapping, genomic context and hydropathy

Q7VZI5 is 36% identical to the three PrpF proteins (which are 78-80% identical to each other, including B. pertussis BP2370) and lacks TIGR02334. It is 35-45% identical to all other characterised SF2 and SF3 members.
Q7VZI5 conserves the PrpF catalytic Lys/Cys pair (K65, C99). It has arginine at the PrpF K281 carboxylate-binding position.
BP0922 lies outside the prp cluster (BP2368-BP2371), next to an MmgE/PrpD-family protein (BP0918) and a succinate-semialdehyde dehydrogenase (BP0919).