Pnkd: exact selected product and same-gene reference

Selected B4F7D2 has 369 residues; reference D3ZXB0 has 424 residues.

Biopython PairwiseAligner global BLOSUM62, gap open -10, extension -0.5.

Reference interval Selected interval Identical residues
1–79 1–79 79
119–379 80–340 251
395–406 341–352 6
408–424 353–369 2

Reference feature mapping (sequence coverage does not establish retained function):

Reference feature Interval Mapped / length Identical
Transmembrane: Helical 74–92 6 / 19 6
Domain: Metallo-beta-lactamase 168–330 163 / 163 163

A shared segment does not establish that a truncated product folds, is expressed, localizes, or catalyzes the full-length reaction.

Inputs are the sibling frozen ProtNLM source and same-gene reference JSON files. Reproduce with UV_NO_SYNC=1 uv run python compare_sequences.py from this directory.